17th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings, Volume II) (2024) [O-06-P, pp. 607-610]
AUTHOR(S) / AUTOR(I): Danijela Z. Krstić 
, Jelena J. Žakula , Lela B. Korićanac , Nada Savić , Tatjana Parac-Vogt and Mirjana B. Čolović
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DOI: 10.46793/Phys.Chem24II.607K
ABSTRACT / SAŽETAK:
This study aimed to investigate the simultaneous effect of Fe(III)-substituted monolacunary Wells-Dawson polyoxometalate (Fe-WD), K7[FeIII(α2-P2W17O61)(H2O)] and L(+)-ascorbic acid sodium salt (ascorbate) on cell viability of human melanoma A375 cells. A375 cells were exposed in vitro to Fe-WD (0.001 – 1 mM), ascorbate (0.040 – 40 mM), and Fe-WD/ascorbate mixtures (concentration ratio 1:40) for 24, 48, and 72 h. Fe-WD and ascorbate inhibited cell viability in a concentration-dependent manner for three treatment durations. Obtained EC50 values were 1, 0.58, and 0.52 mM (for 24-, 48-, and 72-hour exposure, respectively) for Fe-WD and 1.32, 1.12, and 0.80 mM (for 24, 48, and 72 hours, respectively) for ascorbate. The simultaneous exposure to Fe-WD/ascorbate mixtures resulted in cytotoxic effects weaker than the mathematical sum of effects induced by single Fe-WD and ascorbate treatments, indicating an antagonistic inhibitory effect of the studied compounds on A375 cell viability. Conclusively, Fe-WD seems to reduce the cytotoxic effect of sodium ascorbate due to the possible autoxidation of ascorbate by oxygen in the presence of ferric (Fe(III)) ions.
KEYWORDS / KLJUČNE REČI:
ACKNOWLEDGEMENT / PROJEKAT:
This study was funded by the Ministry of Science, Technological Development, and Innovation of the Republic of Serbia (contracts no. 451-03-66/2024-03/200017 and 451-03-9/2023-14/200110). The authors would also like to thank the Science Fund of the Republic of Serbia (POMCACT project no. 6526393).
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