ELECTROCHEMICAL AND EPR CHARACTERIZATION OF STEM CELLS FOR APPLICATIONS IN REGENERATIVE MEDICINE

17th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings, Volume I) (2024) [F-08-P, pp. 239-242]

AUTHOR(S) / АУТОР(И): Đura Nakarada , T. Tomašević, A. Dragićević, Dijana Mitić , Aleksandar Savić and Miloš Mojović

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DOI: 10.46793/Phys.Chem24I.239N

ABSTRACT / САЖЕТАК:

Stem cell research has emerged as a promising course for regenerative medicine. A critical aspect of stem cell applications is the accurate assessment of cell viability and redox status. Traditional methods for evaluating cell viability, such as morphological assessment, cell counting, viability staining, metabolic activity assays, apoptosis detection, and functional assays, are often time- consuming, labor-intensive, and costly. In this study, we explore the use of electrochemical techniques, specifically cyclic voltammetry and electrochemical impedance spectroscopy, to provide a rapid and efficient assessment of dental pulp stem cells (DPSC) viability. Additionally, we employ electron paramagnetic resonance (EPR) spectroscopy to evaluate the redox activity of DPSCs. Our findings suggest that these methods offer an efficient alternative to conventional techniques, potentially enhancing the efficiency of stem cell research and its applications in regenerative medicine.

KEYWORDS / КЉУЧНЕ РЕЧИ:

ACKNOWLEDGEMENT / ПРОЈЕКАТ:

This research has been financially supported by the Ministry of Science, Technological Development and Innovation of Republic of Serbia (Contract No. 451-03-65/2024-03/200146, 451-03-66/2024-03/200146 and 451-03-66/2024-03/200026).

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