Process Optimization of Alcoholic Fermentation for Enhanced Bioethanol Yield

AUTOR(I) / AUTHOR(S): Nevena Vukić , Darko Manjenčić , Marijana Vasić , Milica Ivanović , Predrag Pravdić , Dejan Todorović  and Miodrag Pergal  

 

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DOI: https://doi.org/10.46793/TIE26.329V

SAŽETAK /ABSTRACT:

The high consumption and exhaustion of fossil fuels in recent years due to industry and technology development has led to finding an alternative solution. Among alternative energy resources, biofuels today are a significant place, of which bioethanol has a special place as a renewable and environmentally convenient fuel. In the production of bioethanol, it is significant that it can be obtained by fermentation from renewable raw materials containing carbohydrates. The aim of this study is to determine the optimal combination of microorganism and temperature regime to achieve maximum bioethanol yield, while minimizing energy losses and reducing fermentation time. The presence of the produced bioethanol was confirmed using gas chromatography-mass spectrometry (GC-MS). Additionally, the chemical structure of ethanol and carbohydrates was investigated and verified via Fourier-transform infrared spectroscopy (FTIR), which successfully identified the characteristic functional groups. This paper investigates the effect of temperature on the fermentation process of ethanol production from cassava, using hydrolyzed starch as the carbohydrate source, with yeast cultivated on Sabouraud Maltose Agar. This study thus revealed that a temperature of 30°C and a pH of 4.8 proved most suitable for the fermentation process, resulting in a bioethanol yield of approximately 30% from both glucose and maltose syrups.

KLJUČNE REČI / KEYWORDS: 

Bioethanol; alcoholic fermentation; temperature; ethanol yield; process optimization

PROJEKAT / ACKNOWLEDGEMENT:

This study was supported by the Ministry of Science, Technological Development and Innovation of the Republic of Serbia, and these results are part of the Grant No. 451-03-33/2026-03/200132.

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