Fe(III)-CONTAINING WELLS-DAWSON POLYOXOTUNGSTATE AND ASCORBATE: EFFECT OF EQUIMOLAR MIXTURE ON HELA CELLS

8^th WORKSHOP: FOOD AND DRUG SAFETY AND QUALITY, (pp.155–158)

АУТОР / AUTHOR(S): D. Z. Krstić , M. B. Čolović , L. B. Korićanac , J. J. Žakula , A. Leskovac , N. Savić , T. Parac-Vogt

DOI: 10.46793/8FDSQ.PC6DK

САЖЕТАК / ABSTRACT:

This study aims to explore in vitro anti-tumor action of the equimolar mixture of Fe(III)-substituted monolacunary Wells-Dawson POM (Fe-WD), K₇[Fe^III(α₂-P₂W₁₇O₆₁)(H₂O)] and L(+)-ascorbic acid sodium salt (ascorbate) using cervical carcinoma HeLa cells as a model system.

Exponentially growing HeLa cells were exposed in vitro to Fe-WD and ascorbate separately (0.005 – 0.5 mM) and the equimolar Fe-WD/ascorbate mixtures of the same concentrations for 48 and 72 h, at 37 °C. Single and simultaneous exposure to the studied compounds resulted in concentration- and time-dependent decrease of cell viability. IC₅₀ values for Fe-WD were 0.015 and 0.0073 mM for 48- and 72-hour administration, respectively. Ascorbate induced noticeably weaker cytotoxicity with IC₅₀ values of 0.37 and 0.32 mM for 48 and 72 h, respectively. Simultaneous exposure to the mixtures of Fe-WD and ascorbate demonstrated that the presence of ascorbate did not influence Fe-WD-induced cytotoxicity after the single treatment with the same concentrations for 48 and 72 h.

КЉУЧНЕ РЕЧИ / KEYWORDS:

ПРОЈЕКАТ / ACKNOWLEDGEMENT:

This study was funded by the Ministry of Science, Technological Development, and Innovation of the Republic of Serbia (contracts no. 451-03-66/2024-03/200017 and 451-03-9/2023-14/200110). The authors would also like to thank the Science Fund of the Republic of Serbia (POMCACT project no. 6526393).

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