8th WORKSHOP: FOOD AND DRUG SAFETY AND QUALITY, (pp.127–130)
АУТОР / AUTHOR(S): A. Valenta Šobot 
, D. Drakulić , Đ. Katnić , S. Butulija , M. Janić , A. Krstić , J. Filipović Tričković
DOI: 10.46793/8FDSQ.PB17VS
САЖЕТАК / ABSTRACT:
Gentiana lutea root extract displays protective effects, including activity against oxidative stress, inflammation, and effects on the cardiovascular and central nervous systems. Plant-derived compounds could protect against disease progression by regulating homeostasis and eliminating damaged cells, acting like pro-apoptotic agents. In this work, we aimed to test the impact of cytotoxic concentrations of Gentiana lutea root extract on the activation of apoptosis, a controlled cell death mechanism, in peripheral blood mononuclear cells. Apoptotic DNA degradation was tested by a fragmentation assay. Immunoblot was used to detect proteins Bcl2, Bax, cleaved PARP1, and caspase3 as markers of apoptosis. We detected a high level of DNA fragmentation, an elevation of the Bax, cleaved caspase3, and PARP1 fragments that are indicators of cell death by apoptosis. Gentiana lutea root extract cytotoxic concentration eliminated cells with limited response to xenobiotic exposure by apoptosis.
КЉУЧНЕ РЕЧИ / KEYWORDS:
ПРОЈЕКАТ / ACKNOWLEDGEMENT:
This work was supported by the Serbian Ministry of Science, Technological Development, and Innovation (Grant no. 451-03-66/2024-03/200017).
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