17th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings, Volume II) (2024) [O-07-P, pp. 611-614]
AUTHOR(S) / АУТОР(И): Jelena J. Žakula 
, Mirjana B. Čolović , Lela B. Korićanac , Nada Savić , Tatjana Parac-Vogt and Danijela Z. Krstić
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DOI: 10.46793/Phys.Chem24II.611Z
ABSTRACT / САЖЕТАК:
The objective of this study was to investigate the simultaneous effect of a monolacunary Wells- Dawson polyoxometalate (mono-WD), α2-K10P2W17O61.20H2O, which exhibited remarkable cytotoxicity against HeLa cells, and L(+)-ascorbic acid sodium salt (ascorbate), as a strong antioxidant, against HeLa cells as a commonly used model system for research purposes. HeLa cells were treated in vitro (single exposure) with different concentrations of mono-WD (0.001 – 1 mM) and ascorbate (0.040 – 40 mM) for 24 and 48 hours. In addition, the tumor cells were simultaneously exposed to mixtures containing the same concentrations of mono-WD and ascorbate (ratio 1:40). Mono-WD and ascorbate induced concentration-dependent cytotoxic effects against HeLa cells, with EC50 values of 0.042 and 0.017 mM (for 24 and 48 hours, respectively) for mono- WD and 0.88 and 0.36 mM (for 24 and 48 hours, respectively) for ascorbate. The results of mono- WD/ascorbate mixtures (concentration ratio 1:40) indicated an additive/synergistic cytotoxic effect of the studied compounds against HeLa cell viability. Conclusively, ascorbate improves the antitumour properties of mono-WD as a potential chemotherapeutic candidate.
KEYWORDS / КЉУЧНЕ РЕЧИ:
ACKNOWLEDGEMENT / ПРОЈЕКАТ:
This research was funded by the Ministry of Science, Technological Development, and Innovation of the Republic of Serbia (contracts no. 451-03-66/2024-03/200017 and 451-03-9/2023-14/200110) and the Science Fund of the Republic of Serbia (POMCACT project no. 6526393).
REFERENCES / ЛИТЕРАТУРА:
- B. Čolović, B. Medić, M. Ćetković, T. Kravić Stevović, M. Stojanović, W. W. Ayass, A. S. Mougharbel, M. Radenković, M. Prostran, U. Kortz, Z. Krstić, Toxicol. Appl. Pharm., 333 (2017) 68.
- M. B. Čolović, M. Lacković, J. Lalatović, A. S. Mougharbel, U. Kortz, D. Krstić, Curr. Med. Chem., 27 (2020) 362.
- A. M. Isakovic, M. B. Čolović, T. Ma, X. Ma, M. Jeremić, M. Gerić, G. Gajski, S. Misirlic- Dencic, U. Kortz, Z. Krstić, J. Biol. Inorg. Chem., 26 (2021) 957.
- A. P. Ginsberg, Inorganic Syntheses, John Wiley and Sons, New York, USA, 1990.
- P. Skehan, R. Storeng, D. Scudiero, A. Monks, J. McMahon, D. Vistica, J. T. Warren, H. Bokesch, S. Kenney, M. R. Boyd, JNCI-J. Natl. Cancer I., 82 (1990) 1107.