IN VITRO ANTI-TUMOR EFFECT OF Fe(III)-CONTAINING WELLS-DAWSON POLYANION AND ASCORBATE

8^th WORKSHOP: FOOD AND DRUG SAFETY AND QUALITY, (pp.147–150)

АУТОР / AUTHOR(S): M. B. Čolović , J. J. Žakula , L. B. Korićanac , S. Petrović , N. Savić , D. Z. Krstić , T. Parac-Vogt

DOI: 10.46793/8FDSQ.PC4MC

САЖЕТАК / ABSTRACT:

The cytotoxic effect of the mixture of Fe(III)-substituted monolacunary Wells-Dawson polyoxometalate (Fe-WD), K₇[Fe^III(α₂-P₂W₁₇O₆₁)(H₂O)] and L(+)-ascorbic acid sodium salt (ascorbate) on cervical carcinoma HeLa cells was evaluated in this study. Exponentially growing HeLa cells were treated in vitro with Fe-WD (0.001 – 1 mM), forty times higher concentrations of ascorbate (0.040 – 40 mM), and Fe-WD/ascorbate mixtures (concentration ratio 1 : 40) for 24 h, at 37 °C. Fe-WD and ascorbate inhibited cell viability in a concentration-dependent manner with different potencies. IC₅₀ values were determined as 0.028 and 0.92 mM for Fe-WD and ascorbate, respectively. The simultaneous 24-hour exposure of HeLa cells to Fe-WD/ascorbate mixtures resulted in cytotoxic effects weaker than the mathematical sum of effects induced by single Fe-WD and ascorbate actions at the corresponding concentrations. Thus, it could be considered that Fe-WD and ascorbate act antagonistically on HeLa viability at the concentration ratio 1 : 40. This effect might result from the autoxidation of ascorbate in the presence of Fe(III) ions, resulting in ascorbate and Fe-WD consumption.

КЉУЧНЕ РЕЧИ / KEYWORDS:

ПРОЈЕКАТ / ACKNOWLEDGEMENT:

This study was financially supported by the Ministry of Science, Technological Development, and Innovation of the Republic of Serbia (contracts no. 451-03-66/2024-03/200017 and 451-03-9/2023-14/200110). The authors would also like to thank the Science Fund of the Republic of Serbia (POMCACT project no. 6526393).

ЛИТЕРАТУРА / REFERENCES:

[1] A. M. Isakovic, M. B. Čolović, T. Ma, X. Ma, M. Jeremić, M. Gerić, G. Gajski, S. Misirlic-Dencic, U. Kortz, D. Z. Krstić, J. Biol. Inorg. Chem. 2021, 26, 957.

[2] M. B. Čolović, M. Lacković, J. Lalatović, A. S. Mougharbel, U. Kortz, D. Krstić, Curr. Med. Chem. 2020, 27, 362.

[3] S. A. M. Abdelhameed, F. de Azambuja, T. Vasović, N. D. Savić, T. Ćirković Veličković, T. N. Parac-Vogt, Nat. Commun. 2023, 14, 486.

[4] A. P. Ginsberg, Inorganic Syntheses, John Wiley and Sons, New York, USA, 1990.

[5] P. Skehan, R. Storeng, D. Scudiero, A. Monks, J. McMahon, D. Vistica, J. T. Warren, H. Bokesch, S. Kenney, M. R. Boyd, JNCI-J. Natl. Cancer I. 1990, 82, 1107.