Total phenolic content, antioxidant capacity, and antimicrobial activity of Origanum heracleoticum L., extracted with different solvents

Chemia Naissensis Volume 5, No.2 (2023) (стр. 36-49) 

АУТОР(И) / AUTHOR(S): Jovana D. Ickovski, Ivan R. Palić, Aleksandra S. Đorđević, Vesna P. Stankov Jovanović, Violeta D. Mitić and Gordana S. Stojanović

Е-АДРЕСА / E-MAIL: jovana.ickovski@pmf.edu.rs

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DOI: 10.46793/ChemN5.2.36I

САЖЕТАК / ABSTRACT:

This research was based on the examination of the total phenolic content, antioxidant and antimicrobial activities of hexane, diethyl ether, ethyl acetate, and methanol extracts of Origanum heracleoticum L. grown in Serbia. The antimicrobial activity was determined against five bacteria and two fungi using the disk diffusion method. The total phenolic content of O. heracleoticum solvent extracts was determined and five different tests were used for screening of the antioxidant capacity. The highest total phenolic content was found in ethyl acetate extract (848.48 μg GAE/mg dry extract) and methanol extract (733.43 μg GAE/mg dry extract). The examination of antioxidant activity showed that methanol and ethyl acetate extracts had the strongest activity. The highest correlation was found between DPPH and FRAP (R2 = 0.99), as well as DPPH and CUPRAC (R2= 0.96) assays. The ABTS test was highly correlated with the FRAP test (R2 = 0.95). The antimicrobial assay proved that each extract had an effect against all bacteria and fungi, except against the bacterium Pseudomonas aeruginosa. The highest antibacterial activities were found for methanol extract and ethyl acetate extract against Staphylococcus aureus. The highest antifungal activity was observed for the ethyl acetate extract against both Candida albicans and Aspergillus brasiliensis.

КЉУЧНЕ РЕЧИ / KEYWORDS:

Origanum heracleoticum, total phenolic content, antioxidant capacity, antimicrobial activity

ЛИТЕРАТУРА / REFERENCES:

  • Apak, R., Güçlü, K., Özyürek, M., & Karademir, S.E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52, 7970-7981.
  • Baricevic, D. (1996). Experiences with oregano (Oreganum spp.) in Slovenia, in: Proceedings of the IPGRI International Workshop on Oregano, CIHEAM, Valenzano, Bari, Italy.
  • Baycheva, S. K., Karamalakova, Y. D., Nikolova, G. D., Dobreva, K. Z., & Gadjeva, V. G. (2020). An ethanol extract ability of cultivated white oregano (Origanum heracleoticum L.) of Bulgarian flora to attenuate oxidative stress effects formed under short-term UV-B radiation. Bulgarian Chemical Communications, 52, 118-124.
  • Benzie, I. F. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “Antioxidant Power”: The FRAP assay. Analytical Biochemistry, 239, 70-76.
  • Biondi, D., Cianci, P., Geraci, C., Ruberto, G., & Piattelli, M. (1993). Antimicrobial activity and chemical composition of essential oils from sicilian aromatic plants. Flavour and Fragrance Journal, 8, 331–337.
  • Bocchini, P., Russo, M., & Galletti, G.C. (1998). Pyrolysis-gas chromatography/mass spectrometry used as a microanalytical technique for the characterization of Origanum heracleoticum from Calabria, southern Italy. Rapid Communications in Mass Spectrometry, 12, 1555-1563.
  • Carrubba, A., & Calabrese, I. (1998). Antioxidant compounds in some herbaceous aromatic plants. Acta Horticulturae, 457, 85-93.
  • Conforti, F., Marrelli, M., Menichini, F., Tundis, R., Statti, G. A., Solimene, U., & Menichini, F. (2011). Chemical composition and protective effect of oregano (Origanum heracleoticum L.) ethanolic extract on oxidative damage and on inhibition of NO in LPS-stimulated RAW 264.7 macrophages. Journal of Enzyme Inhibition and Medicinal Chemistry, 26(3), 404-411.
  • De Martino, L., De Feo, V., Formisano, C., Mignola, E., & Senatore, F. (2009). Chemical composition and antimicrobial activity of the essential oils from three chemotypes of Origanum vulgare L. ssp. hirtum (Link) letswaart growing wild in Campania (Southern Italy). Molecules, 14, 2735-2746.
  • De Souza, E.L., de Barros, J.C., da Conceição, M.L., Gomes Neto, N.J.G., & da Costa, A.C.V. (2009). Combined application of Origanum vulgare L. Essential oil and acetic acid for controlling the growth of Staphylococcus aureus in foods. Brazilian journal of Microbiology, 40, 387-393.
  • Exarchou, V., Nenadis, N., Tsimidou, M., Gerothanassis, I.P., Troganis, A., & Boskou, D. (2002). Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage, and summer savory. Journal of Agricultural and Food Chemistry, 50, 5294–5299.
  • Hatano, T., Kagawa, H., Yasuhara, T., & Okuda, T. (1988). Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chemical and Pharmaceutical Bulletin, 36, 2090–2097.
  • Kokkini, S., Karousou, R., Dardioti, A., Krigas, N., & Lanaras, T. (1997). Autumn essential oils of Greek oregano. Phytochemistry, 44, 883–886.
  • Lamaison, J.L., Petitjean-Freytet, C., & Carnat, A. (1990). Rosmarinic acid, total hydroxycinnamic derivatives and antioxidant activity of Apiaceae, Borraginaceae and Lamiceae medicinals. Annales Pharmaceutiques Françaises, 48, 103-108.
  • Lamaison, J.L., Petitjean-Freytet, C., Duke, J.A., & Walker, J. (1993). Hydroxycinnamic derivative levels and antioxidant activity in North American Lamiaceae. Plantes Medicinales Et Phytotherapie 26, 143-148.
  • Lawless, J. (2002). The Encyclopaedia of Essential Oils. London: Thorsons.
  • Lawrence, B.M., Terhlne, S.J., & Hocc, J.W. (1974). 4,5-Epoxy-p-Menth-1-ene: A new Constituent of Origanum Heracleoticum. Phytochemistry, 13, 1012-1013.
  • NCCLS, (National Committee for Clinical Laboratory Standards). (1997). M2-A6 Performance standards for antimicrobial disk susceptibility testing; 6th International Supplement, Wayne, PA.
  • Oyaizu, M. (1986). Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44, 307-315.
  • Özcan, M., & Akgül, A. (1995). Capers (Capparis spp.): composition and pickling Product. Workshop – Medicinal and Aromatic Plants, Ege. Univ. Agric. Fac., Bornova-Ýzmir, Turkey.
  • Poulose, A.J., & Croteau, R. (1978). Biosynthesis of aromatic monoterpenes: Conversion of γ- terpinene to p-cymene and thymol in Thymus vulgaris L. Archives of Biochemistry and Biophysics, 187, 307-314.
  • Re, R., Pellegrini, N., Proreggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231-1237.
  • Rencher, A.C. (2003). Methods of multivariate analysis. (Vol. 492). John Wiley & Sons.
  • Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152-178.
  • Sivropoulou, A., Papanikolaou, E., Nikolaou, C., Kokkini, S., Lanaras, T., & Arsenakis, M. (1996). Antimicrobial and cytotoxic activities of Origanum essential oils. Journal of Agricultural and Food Chemistry, 44(5), 1202-1205.
  • Tsimogiannis, D., Stavrakaki, M., & Oreopoulou, V. (2006). Isolation and characterisation of antioxidant components from oregano (Origanum heracleoticum). International Journal of Food Science & Technology, 41, 39-48.
  • Vokou, D., Kokkini, S., & Bessière, J.-M. (1993). Geographic variation of Greek oregano (Origanum vulgare ssp. hirtum) essential oils. Biochemical Systematics and Ecology, 21, 287-295.
  • Zheng, Z. L., Tan, J. Y. W., Liu, H. Y., Zhou, X. H., Xiang, X., & Wang, K. Y. (2009). Evaluation of oregano essential oil (Origanum heracleoticum L.) on growth, antioxidant effect and resistance against Aeromonas hydrophila in channel catfish (Ictalurus punctatus). Aquaculture, 292(3-4), 214-218.
  • Zhou, K., & Yu, L. (2004). Effects of extraction solvent on wheat bran antioxidant activity estimation. Food Science and Technology, 37, 717-721.