SESELI RIGIDUM WALDST. & KIT: SECONDARY METABOLITES AND ANTIOXIDANT ACTIVITY OF METHANOL, ETHYL ACETATE AND ACETONE EXTRACTS

2nd International Symposium On Biotechnology (2024),  [263-268]

AUTHOR(S) / АУТОР(И): Gorica Djelic, Vesna Veličković, Milica Pavlović

E-MAIL: gorica.djelic@kg.ac.rs

Download Full Pdf   

DOI: 10.46793/SBT29.34GDJ

ABSTRACT / САЖЕТАК:

Representatives of the Seseli genus are recognized for their biological activities. The objective of this study was to analyze the phenolic and flavonoid content, along with the antioxidant effects, of methanol, ethyl acetate, and acetone extracts obtained from the roots, leaves, stems, and fruits of S. rigidum. Through spectrophotometric measurements of phenols and flavonoids, the results revealed that the polar (methanol) solvent extract from the leaves exhibited the highest concentrations of phenols (98.66 ± 2.64 mg GA/g) and flavonoids (20.74 ± 0.55 mg RU/g), surpassing those obtained from the stem. Specifically, the methanol extract from the leaves demonstrated the highest antioxidant activity at 25.87 ± 0.05 μg/mL, while the fruit extract exhibited the lowest antioxidant activity at 638.5 ± 0.01 μg/mL. Notably, the total phenol content displayed a strong positive correlation with the antioxidant activity of the extracts, unlike the total flavonoid content.

KEYWORDS / КЉУЧНЕ РЕЧИ:

S. rigidum, phenols, flavonoids, DPPH

REFERENCES / ЛИТЕРАТУРА:

  • Abbaskhan A., Choudhary M.I., Ghayur M.N., Parween Z., Shaheen F., Gilani A.U. (2012). Biological activities of Indian celery, Seseli diffusum (Roxb. ex Sm.) Sant. & Wagh., Phytotherapy Research, 26, 783-6.
  • IBM CORP. RELEASED (2012). IBM SPSS Statistics for Windows, Version 21.0. Armonk, NY: IBM Corp.
  • Gonçalves M.J., Tavares A.C., Cavaleiro C., Cruz M.T., Lopes M.C., Canhoto J. (2012) Composition, antifungal activity and cytotoxicity of the essential oils of Seseli tortuosum L. and Seseli montanum subsp. peixotoanum (Samp.) M. Laínz from Portugal, Industrial Crops and Products, 39, 204-9.
  • Ilić D.M. (2017). Hemijski sastav, antioksidativna, antimikrobna i antiholinesterazna aktivnost biljnih vrsta Seseli rigidum i Seseli pallasii. Univerzitet u Nišu, Prirodno-matematički fakultet, Niš, 79.
  • Maleki M., Yousefi., Asadi M.H., Mirtadzadini S.M. (2023). Libanotis transcaucasica Schischk Leaf and Seed Ethanolic and Methanolic Extracts: Cytotoxic Activity on MCF7 Cell Line and Antioxidant Properties, Journal of Medicinal Plants and By-products, doi.10.22034/JMPB.2023.362345.1562.
  • Marčetić M., Božić D., Milenković M., Lakušić B., Kovačević N. (2012). Chemical composition and antimicrobial activity of essential oil of different parts of Seseli rigidum, Natural Product Communications, 7(8), 1091-4.
  • Marčetić M., Božić D., Milenković M., Kovačević M., Kovačević N. (2019). Antifungalna aktivnost etarskog ulja Seseli rigidum Waldst. & Kit. (Api Apiaceae) na rast izolata Candida albicans, Archive of Pharmacy, 69, 67-79.
  • Peter C., Moran A., Ryan, L. (2011). Stability of the total antioxidant capacity and total polyphenol content of 23 commercially available vegetable juices before and after in vitro digestion measured by FRAP, DPPH, ABTS and Folin-Ciocalteu methods, Food Research International 44, 217–224.
  • Quettier-Deleu C., Gressier B., Vasseur J., Dine T., Brunet C., Luyckx M., Cazin M., Cazin J.C., Bailleul F., Trotin F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour, Journal of Ethnopharmacology, 72, 35-42.
  • Takao T., Watanabe N., Yagi I., Sakata K. (1994). A simple screening method for antioxidant and isolation of several antioxidants produced by marine bacteria from fish and shellfish, Bioscience, Biotechnology, and Biochemistry, 58 (10), 1780-1783.
  • Živković L., Čabarkapa A., Marčetić M., Kovačević N., Bajić V., Jovičić S. (2016). Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae) Archives of Biological Sciences, Belgrade, 68 (1), 135-144.