AUTHOR(S) / АУТОР(И): Zvjezdana Marković, Iva Šikuten, Anita Bošnjak Mihovilović, Domagoj Stupić, Željko Andabaka, Valentina Tuščić, Ana Šišak, IvanaTomaz, Darko Vončina, Darko Preiner
E-MAIL: zmarkovic@agr.hr
DOI: 10.46793/SBT29.21ZM
ABSTRACT / САЖЕТАК:
This study determines the in vitro influence of viruses (GLRaV-1, GLRaV-3, GFLV, GVA) and the position of meristems on initial shoots on the growth and development of ‘Plavac Mali’ and ‘Pošip’ grapevine cultivars. Infected clones were inoculated on MS medium with 0.5 mg l1-1 benzylaminopurine (BAP) and 0.05 mg l-1 indole-3-acetic-acid (IAA). The positions of meristems were: a – 1st; b – 2nd and 3rd; and c – 4th and 5th. The height and number of nodes, as well as the size of callus, were measured four and eight weeks after inoculation. Clone, position of explants, and sanitary status show no significant influence on tested parameters, except in callus formation, where shoot apex formed the lowest callus. Results revealed that axillary buds on the shoot of grapevine, infected with economically important viruses, can be used for biotechnology purposes.
KEYWORDS / КЉУЧНЕ РЕЧИ:
grapevine, multiplication of clones, virus infection, position of buds
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