2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023 (531-534)
АУТОР(И) / AUTHOR(S): Petar B. Stanić, Biljana Šmit, Jovana Muškinja, Tanja Soldatović, Marko Ilić, Kristian Pastor, Marijana Ačanski
Е-АДРЕСА / E-MAIL: petar.stanic@uni.kg.ac.rs
DOI: 10.46793/ICCBI23.531S
САЖЕТАК / ABSTRACT:
In this paper, the retention behavior of thirteen newly synthesized arylidene derivatives of 2-hydamthione was investigated by normal and reversed phases thin-layer chromatography. In normal phase chromatography, thin layers of silica gel and cyano-propyl (CN) silica gel with fluorescent indicator F254 were used as the stationary phase. As the mobile phase, binary systems of non-aqueous solvents benzene-ethyl acetate and hexane-ethyl acetate were used, 8:2 (v/v). Reversed phase chromatography was performed using a thin layer of octadecyl (C-18) silica gel with fluorescent indicator F254 as the stationary phase and a binary solvent mixture of acetonitrile-water in the ratio 7.5:2.5 (v/v) as the mobile phase. Observing under a UV lamp, the spots of all tested derivatives were clearly visible. The RF value was determined for each spot. Based on the obtained results the HPLC technique is recommended for further chromatographic examination of the newly synthesized arylidene derivatives of 2-hydamthione, using commercial columns with polar chemically bound phases and both non-aqueous and aqueous eluents.
КЉУЧНЕ РЕЧИ / KEYWORDS:
arylidene 2-thiohidantoin derivatives, TLC, normal phases, reversed phases, retention behaviour
ЛИТЕРАТУРА / REFERENCES:
M.A. Metwally, E. Abdel-Latif, Thiohydantoins: synthetic strategies and chemical reactions, Journal of Sulfur Chemistry 33 (2012) 229-257.
B. Šmit, R.Z. Pavlović, A. Radosavljević-Mihailović, A. Došen, M.G. Ćurčić, D.S. Šeklić, M.N. Živanović, Synthesis, characterization and cytotoxicity of palladium(II) complex of 3-[(2-hydroxy-benzyilydene)-amino]-2-thioxo-imidazolidin-4-one, Journal of the Serbian Chemical Society 78 (2013) 217-227.
M.M. Ačanski, Tečna hromatografija derivata estradiola i estrona-monografija, Tehnološki fakultet, Univerzitet u Novom Sadu, Novi Sad (2007).
M.M. Ačanski, Tečna hromatografija derivata benzimidazola, Tehnološki fakultet, Univerzitet u Novom Sadu, Novi Sad (2010).
M. Ilić, M. Ačanski, K. Pastor, Lj. Popović, S. Jovanović-Šanta, New challenge in the lipophilicity determination and separation of biologically active 16,17-secoesterone derivatives by HPLC –Use of pentafluorophenyl-propyl column, Journal of Liquid Chromatography and Related Technologies 43 (2020) 106-117.
M. Ilić, K. Pastor, J. Marković, Lj. Grbović, S. Jovanović-Šanta, I. Mitrović, Z. Trivunović, M. Ačanski, Feasibility study of separation and purification of bile acid derivatives by HPLC on C18 and F5 columns, Steroids 186 (2022) 109074.