2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023 (399-402)
АУТОР(И) / AUTHOR(S): Bojana V. Pantović, Nevena Lj. Stevanović, Tina P. Andrejević, Darko P. Ašanin, Miloš I. Djuran, Biljana Đ. Glišić
Е-АДРЕСА / E-MAIL: bojana.pantovic@pmf.kg.ac.rs
DOI: 10.46793/ICCBI23.399P
САЖЕТАК / ABSTRACT:
In the present study, we investigated the interactions of three mononuclear gold(III) complexes of the general formula [AuCl3(azole)], azole is clotrimazole (ctz, Au1), tioconazole (tcz, Au2) and voriconazole (vcz, Au3) with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission spectroscopy. A significant decrease in fluorescence intensity of BSA in the presence of the complexes and high values of the calculated binding constants have indicated their good binding affinity toward this model protein. Besides that, the investigated Au1-3 complexes have shown a significant binding affinity toward ct-DNA-EthBr system (EthBr is ethidium bromide, which behaves as an intercalator between DNA base pairs). The best binding affinity towards the studied biomolecules has been shown by Au3 complex, which contains a monodentately coordinated voriconazole. The findings from this study could be important for the further development of metal-based antimicrobials, as well as anticancer agents, with favourable activity and therapeutic profile in respect to the parent organic ligands.
КЉУЧНЕ РЕЧИ / KEYWORDS:
gold(III) complexes, antifungal azoles, DNA interactions, protein interactions
ЛИТЕРАТУРА / REFERENCES:
- S.J. Berners-Price, A. Filipovska., Gold compounds as therapeutic agents for human diseases, Metallomics, 3 (2011) 863-873.
- B.Đ. Glišić, M.I. Djuran., Gold complexes as antimicrobial agents: an overview of different biological activities in relation to the oxidation state of the gold ion and the ligand structure, Dalton Transactions, 43 (2014) 5950-5969.
- N.Lj. Stevanović, J. Kljun, I. Aleksic, S. Skaro Bogojevic, D. Milivojevic, A. Veselinovic, I. Turel, M. I. Djuran, J. Nikodinovic-Runic, B. Đ. Glišić., Clinically used antifungal azoles as ligands for gold(III) complexes: the influence of the Au(III) ion on the antimicrobial activity of the complex, Dalton Transactions, 51 (2022) 5322-5334.
- H. Zhang, Y. Wang, H. Zhu, Z. Fei, J. Cao., Binding mechanism of triclocarban with human serum albumin: Effect on the conformation and activity of the model transport protein, Journal of Molecular Liquids, 247 (2017) 281-288.
- A. Tarushi, E. Potatoglou, J. Kljun, I. Turen, G. Psomas, D. P. Kessissoglou., Interaction of Zn(II) with quinolone drugs: Structure and biological evaluation, Dalton Transactions, 40 (2011) 9461-9473.
- Y. Shi, C. Guo, Y. Sun, Z. Liu, F. Xu, Y. Zhang, Z. Wen, Z. Li, Interaction between DNA and Microcystin-LR Studied by Spectra Analysis and Atomic Force Microscopy, Biomacromolecules 12 (2011) 797-803.