2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023 (61-66)
АУТОР(И) / AUTHOR(S): Marijana Petković, Milutin Stepić, Iva Popović, Manuel Algarra, Tanja Dučić, Anamarija Abu el Rub, Vanja Ralić, Lela Korićanac, Jelena Žakula, Jelena Filipović Tričković, Ana Valenta Šobot, Maja D. Nešić
Е-АДРЕСА / E-MAIL: petkovic.marijana.71@gmail.com
DOI: 10.46793/ICCBI23.061P
САЖЕТАК / ABSTRACT:
Small, carbon-based nanoparticles, carbon dots (CDs), are investigated as photosensitizers (PSs) for photodynamic therapy of cancers. The results presented here show biochemical changes in cancer cells treated with surface-modified CDs, which are augmented by illumination, indicating a high potential of CDs as selective drug carriers and potent PSs. In addition, we will show that CDs produced from black carrot extract (biomass) can be exploited for image-guided therapy and be cytotoxic for cancer cells, thanks to their fluorescence properties. Finally, since biomass-fabricated CDs interact strongly with nucleic acids, they can be further developed for cell cycle sensing purposes.
КЉУЧНЕ РЕЧИ / KEYWORDS:
Carbon dots, Photodynamic therapy, cancer, theranostic, biomass
ЛИТЕРАТУРА / REFERENCES:
- J. Liu, R. Li, B. Yang., Carbon Dots: A New Type of Carbon-Based Nanomaterial with Wide Applications, ACS Central Science, 6 (2020) 2179–2195.
- P.-C. Hsu, P.-C. Chen, C.-M. Ou, H.-Y. Chang, H.-T. Chang, Extremely high inhibition activity of photoluminescent carbon nanodots toward cancer cells, Journal of Materials Chemistry B, 1 (2013) 1774.
- M. Lan, G. Liang, Z. Shaojing, Z. Zhenyu, J. Qingyan, Y. Li, X. Jing, Z. Hongyan, W. Pengfei, Z. Wenjun, Carbon Dots as Multifunctional Phototheranostic Agents for Photoacoustic/Fluorescence Imaging and Photothermal/Photodynamic Synergistic Cancer Therapy, Advanced Therapeutics, 1 (2018) 1800077.
- B. B. Campos, R. Contreras-Cáceres, T.J. Bandosz, J. Jiménez-Jiménez, E. Rodríguez-Castellón, J.C.G.E. da Silva, M. Algarra,, Fluorescent chemosensor for pyridine based on N-doped carbon dots, Journal of Colloid and Interface Science, 458 (2015) 209–216.
- L. Cui, X. Ren, M. Sun, H. Liu, L. Xia, Carbon Dots: Synthesis, Properties and Applications, Nanomaterials, 11 (2021) 3419.
- P. Pierrat, R. Wang, D. Kereselidze, M. Lux, P. Didier, A. Kichler, F. Pons, L. Lebeau, Efficient in vitro and in vivo pulmonary delivery of nucleic acid by carbon dot-based nanocarriers, Biomaterials, 51 (2015) 290–302.
Y. K. Jung, E. Shin, B.-S. Kim, Cell Nucleus-Targeting Zwitterionic Carbon Dots, Scientific Reports, 5 (2015) 18807. - M. D. Nešić, T. Dučić, M. Gonçalves, M. Stepić, M. Algarra, J. Soto, B. Gemović, T.J. Bandosz, M. Petković, Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex, Chemico Biological Interactions, 360 (52022) 109950.
- I. Yousef, L. Ribó, A. Crisol, I. Šics, G. Ellis, T. Ducic, M. Kreuzer, N. Benseny-Cases, M. Quispe, P. Dumas, S. Lefrançois, T. Moreno, G. García, S. Ferrer, J. Nicolas, M.A.G. Aranda, MIRAS: The Infrared Synchrotron Radiation Beamline at ALBA, Synchrotron Radiation News, 30 (2017) pp. 4–6.
- H. Alhazmi, FT-IR Spectroscopy for the Identification of Binding Sites and Measurements of the Binding Interactions of Important Metal Ions with Bovine Serum Albumin, Scientia Pharmaceuthica, 87 (2019) 5.
- S. Sirikwanpong, D. Dahlan, S. Ngamukote, S. Sangsuthum, S. Adisakwattana, V. Nopponpunth, T. Himathongkam, The Alterations of Erythrocyte Phospholipids in Type 2 Diabetes Observed after Oral High-Fat Meal Loading: The FTIR Spectroscopic and Mass Spectrometric Studies,”Journal of Clinical Biochemistry and Nutrition, 47 (2010) 111–120.
- M. Kreuzer, S. Stamenković, S. Chen, P. Andjus, T. Dučić, Lipids status and copper in a single astrocyte of the rat model for amyotrophic lateral sclerosis: Correlative synchrotron‐based X‐ray and infrared imaging,” Journal of Biophotonics, 13 (2020) e202000069.
- L. Lilge, “Use of Ruthenium Complexes as Photosensitizers in Photodynamic Therapy,” in Ruthenium Complexes, W. R. Browne, A. A. Holder, M. A. Lawrence, J. L. Bullock Jr, L. Lilge, Eds., Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2017, pp. 117–137.
- M. Havrdova, K. Hola, J. Skopalik, K. Tomankova, M. Petr¸ K. Cepe, K. Polakova, J. Tucek, A.B. Bourlinos, R. Zboril, Toxicity of carbon dots – Effect of surface functionalization on the cell viability, reactive oxygen species generation and cell cycle, Carbon, 99 (2016) 238–248.
- M. Havrdová, I. Urbančič, K. Bartoň Tománková, L. Malina, J. Štrancar, A. B. Bourlinos, Self-Targeting of Carbon Dots into the Cell Nucleus: Diverse Mechanisms of Toxicity in NIH/3T3 and L929 Cells, Internatioal Journal of Molecular Sciences, 22 (2021) 5608.
- M. Havrdová, I. Urbančič, K. B. Tománková, L. Malina, K. Poláková, J. Štrancar, A.B. Bourlinos, Intracellular Trafficking of Cationic Carbon Dots in Cancer Cell Lines MCF-7 and HeLa—Time Lapse Microscopy, Concentration-Dependent Uptake, Viability, DNA Damage, and Cell Cycle Profile, International Journal of Molecular Sciences, 23 (2022) 1077.