XXI međunarodna konvencija o kvalitetu JUSK ICQ – 29 – 31. maj 2025, Beograd, (180-189 str.)
AUTOR(I) / AUTHOR(S): Katarina Krsmanović, Ana Ružanović 
, Neda Milinković , Snežana Jovičić
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DOI: 10.46793/XXIJUSK-ICQ.191K
SAŽETAK / ABSTRACT:
Catecholamines are adrenal hormones and neurotransmitters that follow a circadian rhythm, making a 24-hour urine collection the preferred sample. The HPLC method with electrochemical detection is the method of choice for their determination. Since sample stability is crucial for accurate metabolite measurement, this study examined the effects of storage duration and freeze-thaw cycles on catecholamine stability in urine. Stability testing was conducted on six 24-hour urine samples using HPLC (Alliance Waters e2695 Separations Module). Samples were divided into three groups: those stored at -20 °C, aliquots of eluates stored at +4 °C, and five samples subjected to six freeze-thaw cycles. Noradrenaline and adrenaline remained stable in eluates at +4 °C for 14 days, while dopamine levels significantly decreased within 7 days (p<0.05). At -20 °C, noradrenaline and adrenaline remained stable for 14 days, whereas dopamine concentrations were altered. Noradrenaline was stable for two freeze-thaw cycles, but adrenaline and dopamine changed after one. This study results suggest that samples should be processed immediately when possible. If not, they should be stored below -20 °C, avoiding repeated freeze-thaw cycles.
KLJUČNE REČI / KEYWORDS:
Catecholamines, HPLC, Sample storage, Freeze-thaw cycles, Urine
PROJEKAT / ACKNOWLEDGEMENT:
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