17th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings, Volume II) (2024) [S-07-P, pp. 739-742]
AUTHOR(S) / АУТОР(И): A. Lončar, E. Secchi, J. Jimenez Martinez, H. Tabuteau and T. Le Borgne
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DOI: 10.46793/Phys.Chem24II.739L
ABSTRACT / САЖЕТАК:
Bacterial biofilms are communities of bacteria suspended in extracellular substances, which protect the embedded colonies to ensure continued growth. They affect much of human life, colonizing the surfaces of implants, the lungs of cystic fibrosis patients and affecting biogeochemical cycles in the subsurface. Biofilm growth in these complex environments is poorly understood, as the varied nutrient concentrations give rise to chemical gradients, affecting how bacteria organize and mature. One such bacteria is Pseudomonas putida, an aerophilic subsurface organism that colonizes roots of many important agricultural plants. To investigate P. putida biofilm maturation in such conditions, we developed a novel microfluidic device capable of maintaining a stable oxygen gradient over time under flow. Our results suggest that oxygen gradients are correlated to gradients of biomass, indicating that P. putida biofilm growth is strongly connected to oxygen concentration and not a response to oxidative stress. Future experiments would attempt to determine the exact biochemical mechanism responsible for this growth response by utilizing bacterial mutants lacking in various metabolic capabilities.
KEYWORDS / КЉУЧНЕ РЕЧИ:
ACKNOWLEDGEMENT / ПРОЈЕКАТ:
AL, HT and TLB acknowledge that this project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement N°956457.
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