The impact of cadmium on the development of morphological and functional cardiac disruption in zebrafish (Danio rerio)

7th International Congress of Cardionephrology KARNEF (2025) [pp. 257-264]

AUTHOR(S) / АУТОР(И): Nikola Mitovic , Masa Jovanovic, Dragan Ilic, Djordje Kovacevic, Milica Milosevic, Sanjin Kovacevic, Jelena Nesovic Ostojic, Marija S. Stankovic 

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DOI: 10.46793/KARNEF25.268M

ABSTRACT / САЖЕТАК:

Cadmium (Cd) is a non-essential heavy metal with widespread distribution. Due to increasing exposure and intake, its effects are being investigated in relation to various pathological conditions, including liver, kidney, and heart diseases. A correlation has been observed between maternal Cd levels and certain congenital abnormalities, raising questions about its impact on organ development. Zebrafish serve as a valuable model for studying development and, due to genomic homology with humans, for investigating cardiovascular pathology.

KEYWORDS / КЉУЧНЕ РЕЧИ:

cadmium, zebrafish embryos, heart development

REFERENCES / ЛИТЕРАТУРА:

  • Diamond ML, de Wit CA, Molander S, et al. Exploring the planetary boundary for chemical pollution. Environ Int 2015; 78:8-15.
  • Järup L. Hazards of heavy metal contamination. Br Med Bull 2003; 68:167-82.
  • Tchounwou PB, Yedjou CG, Patlolla AK, et al. Heavy metal toxicity and the environment. Exp Suppl 2012; 101:133-164.
  • Rafati Rahimzadeh M, Rafati Rahimzadeh M, Kazemi S, et al. Cadmium toxicity and treatment: An update. Caspian J Intern Med 2017; 8(3):135-145.
  • Genchi G, Sinicropi MS, Lauria G, et al. The Effects of Cadmium Toxicity. Int J Environ Res Public Health 2020; 17(11):3782.
  • Wang P, Chen H, Kopittke PM, et al. Cadmium contamination in agricultural soils of China and the impact on food safety. Environ Pollut 2019; 249:1038-1048.
  • Johnston JE, Valentiner E, Maxson P, et al. Maternal cadmium levels during pregnancy associated with lower birth weight in infants in a North Carolina cohort. PLoS One 2014; 9(10):e109661.
  • Kippler M, Tofail F, Gardner R, et al. Maternal cadmium exposure during pregnancy and size at birth: a prospective cohort study. Environ Health Perspect 2012; 120(2):284-289.
  • Wang F, Fan F, Wang L, et al. Maternal Cadmium Levels During Pregnancy and the Relationship with Preeclampsia and Fetal Biometric Parameters. Biol Trace Elem Res 2018; 186(2):322-329.
  • Zhang H, Reynolds M. Cadmium exposure in living organisms: A short review. Sci Total Environ 2019; 678:761-767.
  • Schoenwolf GC, et al. Larsen’s human embryology. E-book. Elsevier Health Sciences. 2014.
  • Thompson J, Bannigan J. Cadmium: toxic effects on the reproductive system and the embryo. Reprod Toxicol 2008; 25(3):304-15.
  • Nishimura Y, Inoue A, Sasagawa S, Koiwa J, et al. Using zebrafish in systems toxicology for developmental toxicity testing. Congenit Anom (Kyoto) 2016; 56(1):18-27.
  • MacRae CA, Peterson RT. Zebrafish as tools for drug discovery. Nat Rev Drug Discov 2015; 14(10):721-31.
  • Howe K, Clark MD, Torroja CF, et al. The zebrafish reference genome sequence and its relationship to the human genome. Nature 2013; 496(7446):498-503.
  • Rocke J, Lees J, Packham I, et al. The zebrafish as a novel tool for cardiovascular drug discovery. Recent Pat Cardiovasc Drug Discov 2009; 4(1):1-5.
  • Genge CE, Lin E, Lee L, et al. The Zebrafish Heart as a Model of Mammalian Cardiac Function. Rev Physiol Biochem Pharmacol 2016; 171:99-136.
  • Yalcin HC, Amindari A, Butcher JT, et al. Heart function and hemodynamic analysis for zebrafish embryos. Dev Dyn 2017; 246(11):868-880.
  • Pavlidis M, Digka N, Theodoridi A. et al. Husbandry of zebrafish, Danio rerio, and the cortisol stress response. Zebrafish; 10(4), 524-531.

 

  • Yalcin HC, Amindari A, Butcher JT, et al. Heart function and hemodynamic analysis for zebrafish embryos. Dev Dyn 2017; 246(11):868-880.
  • Lee L, Genge CE, Cua M, et al. Functional Assessment of Cardiac Responses of Adult Zebrafish (Danio rerio) to Acute and Chronic Temperature Change Using High-Resolution Echocardiography. PLoS One 2016; 11(1):e0145163.
  • Dash SN, Narumanchi S, Paavola J, et al. Sept7b is required for the subcellular organization of cardiomyocytes and cardiac function in zebrafish. Am J Physiol Heart Circ Physiol 2017; 312(5):H1085-H1095.
  • Adimalla N. Heavy metals pollution assessment and its associated human health risk evaluation of urban soils from Indian cities: a review. Environ Geochem Health 2020; 42(1):173-190.
  • Kaličanin B, Velimirović D. A Study of the Possible Harmful Effects of Cosmetic Beauty Products on Human Health. Biol Trace Elem Res 2016; 170(2):476-84.
  • Schoeters G, Den Hond E, Zuurbier M, et al. Cadmium and children: exposure and health effects. Acta Paediatr Suppl 2006; 95(453):50-4.
  • Yang WY, Zhang ZY, Thijs L, et al. Left Ventricular Structure and Function in Relation to Environmental Exposure to Lead and Cadmium. J Am Heart Assoc 2017; 6(2):e004692.
  • Hudson KM, Belcher SM, Cowley M. Maternal cadmium exposure in the mouse leads to increased heart weight at birth and programs susceptibility to hypertension in adulthood. Sci Rep 2019; 9(1):13553.
  • Prentice RC, Hawley PL, Glonek T, et al. Calcium-dependent effects of cadmium on energy metabolism and function of perfused rat heart. Toxicol Appl Pharmacol 1984; 75(2):198-210.
  • Ferramola ML, Pérez Díaz MF, Honoré SM, et al. Cadmium-induced oxidative stress and histological damage in the myocardium. Effects of a soy-based diet. Toxicol Appl Pharmacol 2012; 265(3):380-9.
  • Berdougo E, Coleman H, Lee DH, et al. Mutation of weak atrium/atrial myosin heavy chain disrupts atrial function and influences ventricular morphogenesis in zebrafish. Development 2003; 130(24):6121-9.
  • Garrity DM, Childs S, Fishman MC. The heartstrings mutation in zebrafish causes heart/fin Tbx5 deficiency syndrome. Development 2002; 129(19):4635-45.
  • Shu X, Cheng K, Patel N, et al. Na, K-ATPase is essential for embryonic heart development in the zebrafish. Development 2003; 130(25):6165-73.
  • Shen J, Wang X, Zhou D, et al. Modelling cadmium-induced cardiotoxicity using human pluripotent stem cell-derived cardiomyocytes. J Cell Mol Med 2018; 22(9):4221-4235.
  • Ozturk IM, Buyukakilli B, Balli E, et al. Determination of acute and chronic effects of cadmium on the cardiovascular system of rats. Toxicol Mech Methods 2009; 19(4):308-17.
  • Chen CY, Zhang SL, Liu ZY, et al. Cadmium toxicity induces ER stress and apoptosis via impairing energy homoeostasis in cardiomyocytes. Biosci Rep 2015; 35(3):e00214.