3rd International Conference on Chemo and BioInformatics, Kragujevac, September 25-26. 2025. (pp. 673-676)
АУТОР(И) / AUTHOR(S): Aleksandar Kocovic, Jovana Bradic, Anica Petrovic, Marko Simic, Vladimir Jakovljevic, Edina Avdovic
Download Full Pdf 
DOI: 10.46793/ICCBIKG25.673K
САЖЕТАК / ABSTRACT:
Gelatin–alginate hydrogels incorporating grape skin extract (GSE) from the Šumadija region were prepared by blending alginate and gelatin (3:1, 3:2, 1:1), casting, freezing, and CaCl₂ cross-linking. Organoleptic properties, total phenolic content (TPC, Folin–Ciocalteu), and antioxidant activity (DPPH and ABTS microplate assays with Trolox) were evaluated. GSE- loaded hydrogels formed uniform, solid gels. Compared with blank matrices, GSE increased TPC by approximately 46–79% and produced modest but consistent reductions in SC50 values: 0.3– 1.8% in ABTS and 0.5–1.6% in DPPH (Trolox 6.909 and 5.034 µg/mL, respectively). Across GSE formulations, higher gelatin fractions correlated with higher TPC and lower ABTS SC50, suggesting protein–polyphenol interactions that enhance aqueous availability, whereas DPPH responses were minimally affected. These findings indicate that GSE incorporation preserves antioxidant functionality and yields small in-assay gains, with composition exerting a stronger effect in ABTS than in DPPH. The platform is a sustainable route to bioactive wound dressings and merits further optimization and biological validation
КЉУЧНЕ РЕЧИ / KEYWORDS:
grape skin, hydrogel, antioxidant, polyphenols
ПРОЈЕКАТ / ACKNOWLEDGEMENT:
This research was supported by the Science Fund of the Republic of Serbia, #GRANT No 11067, Unleashing Nature’s Potential: Using Grape Skin Extract and Sustainable Materials for Advanced Chronic Wound Therapy—GraSP_MAT and #GRANT No 14848, Unleashing nature’s potential: Development of Additive-Free Prokupac Grape Skin Topping as Functional Food—GraSP, and also by the Serbian Ministry of Science, Technological Development, and Innovations Agreements No. 451- 03-137/2025-03/200111, 451-03- 136/2025-03/200111 (assigned to Faculty of Medical Sciences), and Agreements No. 451-03-136/2025- 03/200378, 451-03-137/2025-03/200252, and 451-03-136/2025-03/200122 (assigned to Institute for Information Technologies).
ЛИТЕРАТУРА / REFERENCES:
- P. Lu, D. Ruan, M. Huang, et al., Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions, Signal Transduction and Targeted Therapy, 9 (2024) 166.
- L. Maeso, P.E. Antezana, A.G. Hvozda Arana, P.A. Evelson, G. Orive, M.F. Desimone, Progress in the use of hydrogels for antioxidant delivery in skin wounds, Pharmaceutics, 16 (2024) 524.
- J. Bradic, A. Petrovic, A. Kocovic, V. Ugrinovic, S. Popovic, A. Ciric, Z. Markovic, E. Avdovic, Development and optimization of grape skin extract-loaded gelatin–alginate hydrogels: assessment of antioxidant and antimicrobial properties, Pharmaceutics, 17 (2025) 790.
- V. Gounden, M. Singh, Hydrogels and wound healing: current and future prospects, Gels, 10 (2024) 43.
- M. Hunt, M. Torres, E. Bachar-Wikstrom, et al., Cellular and molecular roles of reactive oxygen species in wound healing, Communications Biology, 7 (2024) 1534.
- T. Magrone, M. Magrone, M.A. Russo, E. Jirillo, Recent advances on the anti-inflammatory and antioxidant properties of red grape polyphenols: in vitro and in vivo studies, Antioxidants, 9 (2019) 35.
- K. Acharya, Simplified methods for microtiter based analysis of in vitro antioxidant activity, Asian Journal of Pharmaceutics, 11 (2017) 327–335.