Targeting Thyroid Cancer: In Vitro Analysis of A Benzofuran-Substituted Chalcone Compound

АУТОР(И) / AUTHOR(S): Elif Erturk, Aysen Sagnak, Ceren Tatarlar, Demet Coskun, Ferda Ari

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DOI:  10.46793/ICCBIKG25.497E

САЖЕТАК / ABSTRACT:

Thyroid cancer, the most common endocrine cancer worldwide, causes serious problems, particularly in women. Chalcones, derived from natural compounds and precursors of flavonoids, are known to hold promise in cancer treatment due to their anticarcinogenic effects in many types of cancer, including thyroid. In the current study, the anticarcinogenic effects of the synthesized 2-benzofuran-linked chalcone compound were investigated in human thyroid cancer cell lines (TPC-1 and 8505-C) and a healthy thyroid cell line (Nhty-Ori 3-1). The effects of the compound on cell viability in TPC-1 and 8505-C cells were examined using SRB assays at 24, 48, and 72 hours, and the SRB viability test findings were confirmed by ATP analysis at the end of the 48-hour treatment period. Triple fluorescent staining (Hoechst 33342 + Annexin-V + Propidium Iodide) was used to determine the cell death pathway responsible for the cytotoxic effects of the chalcone compound. Consequently, the cytotoxic effect of the chalcone compound was observed in TPC-1 and 8505-C cells, while the cytotoxic effect for Nhty-Ori 3-1 was found to be quite low. The triple staining revealed that the chalcone compound induced apoptosis in TPC- 1 and 8505-C cells. Based on these results, it was concluded that the anti-cancer mechanisms of the 2-benzofuran-linked chalcone compound in thyroid cancer should be investigated and further analyses should be conducted.

КЉУЧНЕ РЕЧИ / KEYWORDS:

Thyroid cancer, Chalcone Compound, Anticancer, Apoptosis

ПРОЈЕКАТ / ACKNOWLEDGEMENT:

We would like to thank our Bursa Uludag University Molecular Cancer Research Laboratory (BUMKAL) group for obtaining the data and contributing to the methodology. This study was supported by the 2209-A – University Students Research Projects Support Program (TÜBİTAK) under project number 1919B012407219.

ЛИТЕРАТУРА / REFERENCES:

• D. Hanahan, R.A. Weinberg., Hallmarks of Cancer: The Next Generation, Cell, 144 (2011) 646- 674.
• M. Balcı, M. Eryol., İnsidental Saptanan Mezenterik Kalsifiye Fibröz Tümör: Olgu Sunumu, Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, 23 (2021) 186-190.
• D. Laha, N. Nilubol, M. Boufraqech., New Therapies for Advanced Thyroid Cancer, Front Endocrinol (Lausanne), 22 (2020) 82-88.
• A. Coca-Pelaz, J.P. Shah, J.C. Hernandez-Prera et al., Papillary Thyroid Cancer-Aggressive Variants and Impact on Management: A Narrative Review, Adv Ther, 37 (2020) 3112-3128.
• Z. Pan, T. Xu, L. Bao et al., CREB3L1 Promotes Tumor Growth and Metastasis of Anaplastic Thyroid Carcinoma By Remodeling the Tumor Microenvironment, Mol Cancer, 21 (2022) 190.
• J. Sharifi-Rad, S. Rajabi, M. Martorell et al., Plant natural products with anti-thyroid cancer activity, Fitoterapia, 146 (2020) 104640.
• C. Sezgin, Kanserde Bitkilerle Tedavide Örnek Uygulamalar. Bitkilerle Tedavi Sempozyumu, 172 (2010).
• N. Bashir, S. Hussain, A. Khan et al., Cancer Drug Resistance: A Fleetto Conquer. Journal of Cellular Biochemistry, 120 (2019) 14213-14225.
• X. Shi, W. Xu, H. Yao et al., Dietary Flavonoids As Cancer Prevention Agents. Journal of Environmental Science and Health, 29 (2011) 1-31.
• C. A. Blair, D.D. Jandial, L.S. Krill et al., Molecular targeted approaches to cancer therapy and prevention using chalcones, Current Cancer Drug Targets, 14 (2014), 181-200.
• Y. S. Ku, M.S. Ng, S.S. Cheng et al., Understanding the Composition, Biosynthesis, Accumulation and Transport of Flavonoids in Crops for the Promotion of Crops as Healthy Sources of Flavonoids for Human Consumption, Nutrients, 2020 12(2020) 1717.
• P. Aiello, A. P. Ardekan, A. Bishayee et al., Medicinal Plants in The Prevention and Treatment of Colon Cancer, Oxidative Medicine and Cellular Longevity, 4 (2019) 2075614.
• A. Aiello, M. Casertano, S. Della et al., In Vitro Anti-Proliferative Evaluation of Synthetic Meroterpenes Inspired by Marine Natural Products. Marine Drugs, 17 (2019) 684.
• E. Manzo, Synthesis of Marine Natural Products and Molecules Inspired By Marine Substances. Marine Drugs, 19 (2021) 208.
• L. O. Vajrabhaya, S. Korsuwannawong, Cytotoxicity Evaluation of A Thai Herb Using Tetrazolium (MTT) and Sulforhodamine B (SRB) Assays. J Anal Sci Technol, 15 (2018) 1‐6.
• E. Erturk, O.E. Onur, I. Aydin et al., Targeting The Epithelial-Mesenchymal Transition (EMT) Pathway With Combination of Wnt Inhibitor and Chalcone Complexes in Lung Cancer Cells. J Cell Biochem, 124 (2023) 1203-1219.