3rd International Conference on Chemo and BioInformatics, Kragujevac, September 25-26. 2025. (pp. 457-460)
АУТОР(И) / AUTHOR(S): Tamara M. Mladenović, Dušica M. Simijonović, Edina H. Avdović, Dejan A. Milenković, Sandra Jovičić Milić, Danijela Stojković, Zoran S. Marković
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DOI: 10.46793/ICCBIKG25.457M
САЖЕТАК / ABSTRACT:
The formation of β-cyclodextrin (β-CD) inclusion complexes is a well-established strategy to enhance the solubility, stability, and bioavailability of poorly soluble bioactive compounds. In this study, the in vitro antioxidant potential of an inclusion complex (CPD-β-CD) formed between β-CD and 8,9-dihydroxy-2H-chromeno[2,3-d]pyrimidine-2,4(3H)-dione (CPD) was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. The percentage of radical scavenging activity was assessed for the CPD-β-CD complex, along with standard antioxidants quercetin and nordihydroguaiaretic acid (NDGA), at concentrations of 25 μM, 50 μM, and 100 μM. Notably, the CPD-β-CD complex exhibited strong antioxidant activity, achieving 96.1% inhibition of DPPH radicals even at the lowest tested concentration (25 μM). Furthermore, the IC₅₀ value was determined to be 4.5 μM, indicating a high degree of antiradical potency. These results suggest that the encapsulation of CPD within β-cyclodextrin does not significantly diminish its antioxidant effectiveness. On the contrary, the inclusion complex retains comparable activity to the parent compound, making it a promising candidate for further development in antioxidant applications.
КЉУЧНЕ РЕЧИ / KEYWORDS:
Antioxidant Activity, DPPH radical scavenging assay, β-Cyclodextrin Inclusion Complex
ПРОЈЕКАТ / ACKNOWLEDGEMENT:
This research is funded by the Ministry of Education and Ministry of Science, Technological Development and Innovation, Republic of Serbia, No. 451-03-136/2025- 03/200378.
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