In Vivo Models for Spinal Disc Regeneration

AUTOR(I) / AUTHOR(S): Mihailo Jovanovic, Marija Brankovic, Nenad Grujovic, Fatima Zivic

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DOI:  10.46793/ICCBIKG25.420J

SAŽETAK / ABSTRACT:

Intervertebral disc (IVD) degeneration is one of the common health conditions related to pain in lower back, representing one of the major reasons for disabilities from musculoskeletal problems. Spinal disc replacement and regeneration is a recognised approach in clinical approaches, with several commercially available implants. However, better fitted implants and customised solutions are still needed. This paper presents a short review of the most important aspects for appropriate selection and application of different in vivo models for intervertebral disc (IVD) degeneration. Techniques for inducing disc degeneration in models are elaborated, including needle puncture models, enzymatic or chemical induction, genetic and age-related models and mechanical loading and instability models. Different assessment and evaluation methods in in vivo studies are analysed, such as imaging techniques, histological analysis, biomechanical testing and molecular and cellular assays. Current strategies for disc regeneration that have been tested in vivo are presented, including biomaterial scaffolds and hydrogels, cell- based therapies and translational considerations.

KLJUČNE REČI / KEYWORDS:

Intervertebral disc, In vivo models, Bioimaging, Organ-on-chip

PROJEKAT / ACKNOWLEDGEMENT:

This paper is supported through the EIT’s Higher Education Initiative SMART-2M, DEEPTECH-2M and A-SIDE projects, coordinated by EIT RawMaterials, funded by the European Union and the i-GREENPHARM project, HORIZON-MSCA-2023-SE-01-01, Grant No. 101182850 and supported by the Ministry of Education and Ministry of Science, Technological Development and Innovation, Republic of Serbia, Grants: No. 451-03-137/2025-03/200107 and 451-03-136/2025-03/200378.

LITERATURA / REFERENCES:

• M. Baldia, S. Vasnik, Bio-inspired and Biomimetic Materials/Architecture in Intervertebral Disc Regeneration, in: F. Ghorbani, B. Ghalandari, C. Liu (Eds.), Principles of Bioinspired and Biomimetic Regenerative Medicine, Springer Nature Switzerland, Cham, 2025: 657–697. https://doi.org/10.1007/978-3-031-87744-5_18.
• J. Y. Hong, H. Kim, W.-J. Jeon, C. Yeo, H. Kim, J. Lee, Y.J. Lee, I.-H. Ha, Animal Models of Intervertebral Disc Diseases: Advantages, Limitations, and Future Directions, Neurology International 16 (2024) 1788–1818. https://doi.org/10.3390/neurolint16060129.
• P. Zhu, F. Kong, X. Wu, Z. Dong, J. Du, Y. Mao, H. Zhou, Y. Liu, H. Mao, Y. Gu, H. Yang, Geng, A Minimally Invasive Annulus Fibrosus Needle Puncture Model of Intervertebral Disc Degeneration in Rats, World Neurosurgery 169 (2023) e1–e8. https://doi.org/10.1016/j.wneu.2022.09.062.
• G. Tripathi, L. Guha, H. Kumar, Seeing the unseen: The role of bioimaging techniques for the diagnostic interventions in intervertebral disc degeneration, Bone Reports 22 (2024) 101784. https://doi.org/10.1016/j.bonr.2024.101784.
• A.L.A. Binch, J.C. Fitzgerald, E.A. Growney, F. Barry, Cell-based strategies for IVD repair: clinical progress and translational obstacles, Nat Rev Rheumatol 17 (2021) 158–175. https://doi.org/10.1038/s41584-020-00568-w.
• Y. S. Zhang, J. Aleman, S.R. Shin, T. Kilic, D. Kim, S.A. Mousavi Shaegh, S. Massa, R. Riahi, S. Chae, N. Hu, H. Avci, W. Zhang, A. Silvestri, A. Sanati Nezhad, A. Manbohi, F. De Ferrari, A. Polini, G. Calzone, N. Shaikh, P. Alerasool, E. Budina, J. Kang, N. Bhise, J. Ribas, A. Pourmand, A. Skardal, T. Shupe, C.E. Bishop, M.R. Dokmeci, A. Atala, A. Khademhosseini, Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors, Proc. Natl. Acad. Sci. U.S.A. 114 (2017). https://doi.org/10.1073/pnas.1612906114.
• T. Rudroff, Artificial Intelligence as a Replacement for Animal Experiments in Neurology: Potential, Progress, and Challenges, Neurology International 16 (2024) 805–820. https://doi.org/10.3390/neurolint16040060.