3rd International Conference on Chemo and BioInformatics, Kragujevac, September 25-26. 2025. (pp. 288-292)
АУТОР(И) / AUTHOR(S): Sanja Goč, Zorana Dobrijević, Tamara Janković, Filip Janjić, Ninoslav Mitić, Miroslava Janković
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DOI: 10.46793/ICCBIKG25.288G
САЖЕТАК / ABSTRACT:
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality worldwide, largely due to limited tools for early detection and the asymptomatic nature of early- stage disease. Circulating microRNAs (miRNAs) have emerged as promising non‐invasive biomarkers because of their high stability in body fluids and disease‐specific expression patterns. In this study, we performed an in silico analysis of serum miRNA expression profiles using the Gene Expression Omnibus (GEO) dataset GSE113740, which included 40 patients with HCC and 10 healthy controls. A total of 257 differentially expressed miRNAs were identified, of which seven (hsa‐miR‐885‐3p, hsa‐miR‐320a, hsa‐miR‐744‐5p, hsa‐miR‐221‐3p, hsa‐miR‐561‐3p, hsa‐miR‐124‐3p, and hsa‐miR‐637) were prioritized based on network metrics, including the number of single‐line regulators (NSR) and transcription factor percentage (TFR) (p < 0.05). The majority have previously been reported as functionally associated with HCC. Meta‐profile analysis across 40 cancer types revealed distinct liver cancer‐specific expression for five of these miRNAs (hsa-miR-885-3p, hsa-miR-320a, hsa-miR-744-5p, has-miR-221-3p, and has-miR-124-3p). Network topology and pathway enrichment highlighted key regulatory hubs and cancer‐related targets. Ultimately, four miRNAs (hsa‐miR‐885‐3p, hsa‐miR‐320a, hsa‐miR‐744‐5p, and hsa‐miR‐124‐3p) emerged as promising diagnostic biomarkers for early HCC detection.
КЉУЧНЕ РЕЧИ / KEYWORDS:
hepatocellular carcinoma, microRNA, biomarker
ПРОЈЕКАТ / ACKNOWLEDGEMENT:
This research is funded by the Ministry of Science, Technological Development and Innovation, Republic of Serbia, Contract No: 451-03-136/2025-03/200019.
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