3rd International Conference on Chemo and BioInformatics, Kragujevac, September 25-26. 2025. (pp. 280-283)
АУТОР(И) / AUTHOR(S): Marijana D. Janić, Mila N. Kostić, Ksenija M. Radošević, Ana P. Valenta Šobot, Jelena G. Filipović Tričković, Tatjana G. Momić
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DOI: 10.46793/ICCBIKG25.280J
САЖЕТАК / ABSTRACT:
It was observed that ethyl methanesulfonate (EMS) shows DNA alkylating capabilities. The aim of this study was to validate the in silico prediction of EMS genotoxicity obtained by the VEGA-QSAR platform with the micronuclei frequency and proliferation potential from in vitro testing on human PHA-stimulated peripheral blood mononuclear cells (PBMCs). Mitogen- stimulated PBMCs were a model system for EMS genotoxicity testing in cytokinesis-block micronucleus (CBMN) assay. The observed in vitro results had the highest agreements with both in silico IRFMN-VERMEER 1.0.1 and IRFMN 1.0.2 prediction models.
КЉУЧНЕ РЕЧИ / KEYWORDS:
Ethyl methanesulfonate, in silico, genotoxicity, micronucleus, mononuclear cells
ПРОЈЕКАТ / ACKNOWLEDGEMENT:
This research is funded by the Ministry of Education and the Ministry of Science, Technological Development and Innovation, Republic of Serbia, Grant: No. 451-03- 136/2025-03/ 200017.
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