Enhanced polyphenolic extraction efficiency and antioxidant activity of sage using a deep eutectic solvent based on choline chloride and urea

3rd International Conference on Chemo and BioInformatics, Kragujevac, September 25-26. 2025. (pp. 249-252) 

 

АУТОР(И) / AUTHOR(S): Marijana D. Janić, Jelena G. Filipović Tričković, Ana P. Valenta Šobot, Ksenija M. Radošević, Mila N. Kostić, Tatjana G. Momić

 

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DOI:  10.46793/ICCBIKG25.249J

САЖЕТАК / ABSTRACT:

Traditional medicine has utilized sage to treat and prevent a wide range of illnesses. The aim of this study was to determine and compare the polyphenol content and antioxidative activity of sage leaves extracts made with choline chloride: urea mixed with 50% water (ChCl:U) and pure sage water extract (dH2O). Total phenolic content (TPC), total tannin content (TTC), and total flavonoid content (TFC) in extracts were determined. Radical scavenging capacity of sage extracts was measured using the DPPH and ABTS assays. It was demonstrated that the polarity of the solvent had distinct impacts on the extraction of polyphenols from sage leaves using the identical extraction process with ChCl:U and water. Higher polyphenol content (TPC and TTC) and radical scavenging properties against DPPH• and ABTS+• were observed in sage extract made with ChCl:U. TFC in both extracts was almost equal. Results indicated that choline chloride-based DES is an efficient solvent for sage leaf extraction as compared to water.

КЉУЧНЕ РЕЧИ / KEYWORDS:

sage, DES, polyphenols, antioxidant activity

ПРОЈЕКАТ / ACKNOWLEDGEMENT:

This research is funded by the Ministry of Science, Technological Development and Innovation, Republic of Serbia, Grant: No. 451-03-136/2025-03/ 200017.

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