3rd International Conference on Chemo and BioInformatics, Kragujevac, September 25-26. 2025. (pp. 152-155)
АУТОР(И) / AUTHOR(S): Nađa Grozdanić, Nikolina Sibinčić, Ljubodrag Aleksić, Aleksandar Ivanov, Lora Tubić, Nikola Gligorijević, Marija Stojadinović, Simeon Minić
Download Full Pdf 
DOI: 10.46793/ICCBIKG25.152G
САЖЕТАК / ABSTRACT:
The high cost and ethical concerns surrounding fetal bovine serum (FBS) necessitate the development of sustainable alternatives for cellular agriculture, with algal-derived hydrolysates emerging as promising candidates. This study screened the potential of hydrolysates from algae Porphyra haitanensis and Porphyridium purpureum as well as purified chromopeptides from the cyanobacterium Arthrospira platensis to support quail myoblast (QM7) proliferation in a low-serum (1% FBS) medium. Hydrolysates were produced via enzymatic digestion of phycobiliproteins, and chromopeptides were isolated by HPLC. Their effect on QM7 viability was measured after 72 hours using a resazurin assay. Results showed that hydrolysates from A. platensis were highly effective growth promoters, with the chymotrypsin digest increasing cell viability to approximately 160%. Individual purified fractions from A. platensis were also biocompatible. These tested chromopeptides have a good potencial as a cost- effective, and sustainable alternative to FBS for applications in cellular agriculture.
КЉУЧНЕ РЕЧИ / KEYWORDS:
chromopeptides, cellular agriculture, algae, phycobiliproteins
ПРОЈЕКАТ / ACKNOWLEDGEMENT:
This research was supported by the Science Fund of the Rupublic of Serbia, Grant No 10302, Exploring the potential of algal-derived (chromo)peptides as serum replacement components for meat cultivation – PEP4MEAT and Ministry of Education, Science and Technological Development of the Republic of Serbia Agreement No. 451-03-136/2025- 03/200043.
ЛИТЕРАТУРА / REFERENCES:
- M. Guiotto, W. Raffoul, A. Hart, M. Riehle, P. Summa., Human platelet lysate to substitute fetal bovine serum in hmsc expansion for translational applications: a systematic review. Journal of Translational Medicine. 18(1) (2020) 351.
- N. Vasantha, K. Rajagopalan, J. Christyraj, K. Chelladurai, M. Ganesan, Azhagesan, J. Christyraj., Heat‐inactivated coelomic fluid of the earthworm perionyx excavatus is a possible alternative source for fetal bovine serum in animal cell culture. Biotechnology Progress. 35(4) (2019) e2817.
- D. Lanzoni, F. Bracco, F. Cheli, B. Colosimo, D. Moscatelli, A. Baldi, C. Giromini.,. Biotechnological and technical challenges related to cultured meat production. Applied Sciences. 12(13) (2022) 6771.
- P. Kumar, A. Nagarajan, P.D. Uchil. Analysis of Cell Viability by the Alamarblue Assay. Cold Spring Harb. Protoc. (2018) 462–464.