3rd International Conference on Chemo and BioInformatics, Kragujevac, September 25-26, 2025. (pp. 81-84)
АУТОР(И) / AUTHOR(S): Dušan Dimić, Dušica Simijonović, Sandra Jovičić Milić, Edina Avdović
Download Full Pdf 
DOI: 10.46793/ICCBIKG25.081D
САЖЕТАК / ABSTRACT:
The antioxidant and anti-inflammatory properties of grape skin extracts derived from the indigenous Serbian grape variety Prokupac, using ultrasound-assisted extraction (UAE), are showed in this contribution. Extraction was performed with 50% ethanol, absolute ethanol, and ethyl acetate, yielding values between 4.9% and 9.8%. The highest total phenolic content (TPC) was obtained for extracts in 50% ethanol without prior maceration – PSE0 (22.4 mg GAE/g GSE), followed by ethyl acetate – PSE3 (9.8 mg GAE/g GSE), while ethanol-based extracts contained markedly lower phenolic levels. Antioxidant activity, assessed by DPPH, ABTS, and FRAP assays, confirmed that PSE0 exhibited the strongest radical scavenging ability (SC50 = 0.3 µg/mL), comparable to standard antioxidants, whereas PSE3 also showed significant activity. Anti- inflammatory activity, evaluated through lipoxygenase (LOX) inhibition, demonstrated that PSE0 (IC50 = 7.9 µg/mL) outperformed quercetin and approached NDGA, the reference inhibitor. These results highlight grape skins, a winemaking by-product, as a sustainable source of bioactive compounds with potential functional food and nutraceutical applications.
КЉУЧНЕ РЕЧИ / KEYWORDS:
Prokupac grape-skin extracts, antioxidant activity, DPPH, LOX inhibition activity.
ПРОЈЕКАТ / ACKNOWLEDGEMENT:
Financial resources through the Slovenian Research and Innovation Agency (ARIS) project and program grants P2-0438, I0-E015, P1-0403, Z1-50021, L2-4430, J1- 4414, J3-4497, J7-4638, J3-4498, J1-4398, J4-4633, J7-50043, J1-50034, GC-0001, J1-60001, J2-60044, and L7-60161.
ЛИТЕРАТУРА / REFERENCES:
- T. W. Caldas, K. E. L. Mazza, A. S. C. Teles, G. N., Mattos, A. I. S. Brígida, C. A. Conte-Junior, G. Borguini, R. L. O. Godoy, L. M. C. Cabral, R. V. Tonon, Phenolic compounds recovery from grape skin using conventional and non-conventional extraction methods, Industrial Crops and Products, 111 (2018) 86–91.
- E. Avdović, D. Dimić, Đ. Nakarada, D. Simijonović, S. Jovičić Milić, K. Marković, M. Grujović, M. Antonijević, A. Ćirić, D. Milenković, Z. Marković, Evaluation of Bioactive Properties of Ultrasound-Assisted Extracts from Prokupac Grape Skins for Functional Foods, Antioxidants, 14(6) (2025) 733.