Chemia Naissensis Volume 7, No.2 (2025) (стр. 1-21)
АУТОР(И) / AUTHOR(S): Danica Đorđević, Marjan Ranđelović 
Download Full Pdf 
DOI: 10.46793/ChemN7.2.O1DJ
САЖЕТАК / ABSTRACT:
Supercritical carbon dioxide extraction is an advanced separation technique that is increasingly applied in various industries due to its environmental friendliness, selectivity and efficiency. This method enables the extraction of bioactive compounds without the use of toxic chemicals and at lower temperatures compared to classical extraction techniques. This review paper outlines the fundamental principles of supercritical extraction, including the physicochemical properties of supercritical CO2, its advantages over conventional methods, and key process parameters such as pressure, temperature, flow rate, extraction time, and modifiers. Special attention is paid to the industrial applications of this technique, particularly in the pharmaceutical, food, and cosmetic industries, where it is utilised to extract essential oils, antioxidants, polyphenols, lipids, and other valuable components. Moreover, environmental aspects of supercritical extraction were discussed, with an emphasis on sustainability and the potential for minimizing the environmental impact. Through the analysis of current research and technological innovations, this paper provides insight into the perspectives of further development and optimization of supercritical extraction using CO2, as well as its possible application in new industrial areas.
КЉУЧНЕ РЕЧИ / KEYWORDS:
supercritical extraction, carbon dioxide, sustainable technology, separation, bioactive compounds, industrial application
ПРОЈЕКАТ/ ACKNOWLEDGEMENT:
ЛИТЕРАТУРА / REFERENCES:
- Anagha, E.K., Sariga, R., ShyamSundar, K., Panda, C. Rames., Kalavathy, M. Helen., Naik, S. N. (2025). Supercritical CO2 assisted extraction, purification and modelling of free fatty acids from the crude mixture – A case study of tallow hydrolysis. The Journal of Supercritical Fluids, 219,106542.
- Attard, M. T., McElroy, C. R., Gammons, J. R., Slattery, M. J., Supanchaiyamat, N., Alvim Kam,L., Dolstra, O., Trindade, M. L., Bruce, C. N., McQueen-Mason, J. S., Shimizu, S., Hunt, J. A. (2016). Supercritical CO2 Extraction as an Effective Pretreatment Step for Wax Extraction in a Miscanthus Biorefinery ACS Sustainable Chemistry & Engineering, 4(11), 5979-5988.
- Bañares, L. V., Chabni, A. C. (2024). Supercritical fluid technology for lupin hulls valorization: extraction and fractionation of lupeol. Biomass Conv. Bioref. 14, 32453–32463.
- Barbini, S., Sriranganadane, D., España Orozco, S., Kabrelian, A., Karlström, K., Rosenau, T., Potthast, A. (2021). Tools for Bark Biorefineries: Studies toward Improved Characterization of Lipophilic Lignocellulosic Extractives by Combining Supercritical Fluid and Gas Chromatography ACS Sustainable Chem., 9(3), 1323–1332.
- Cassel, E., Frizzo, D. C., Vanderlinde, R., Atti-Serafini, L., Lorenzo, D., Dellacassa, E. (2000). Extraction of Baccharis Oil by Supercritical CO2. Industrial & Engineering Chemistry Research, 39(12), 4803-4805.
- Chabni, A., Bañares, C., Vázquez, L., Torres, F. C. (2025). Combination of expeller and supercritical CO2 for the extraction of a phenolic-rich olive oil – A preliminary chemical characterization. Journal of Industrial and Engineering Chemistry, 147, 755-767.
- Christaki, S., Sulejmanović, M., Simić, S., Kyriakoudi, A., Mourtzinos, I., Vidović, S. (2024). Supercritical CO2 and subcritical water extraction of Curcuma longa bioactive compounds Microchemical Journal, 207, 112101.
- Cruz-Sanchez, E., García-Vargas, J. M., Gracia, I., Rodriguez, J. F., García, M. T. (2024). Supercritical CO2 extraction of lavender flower with antioxidant activity: Laboratory to a large scale optimization process. Journal of the Taiwan Institute of Chemical Engineers, 157, 105404.
- Da Costa Lopes, M. A., Brenner, M., Falé, P., Roseiro, B. L., Bogel-Łukasik, R. (2016). Extraction and Purification of Phenolic Compounds from Lignocellulosic Biomass Assisted by Ionic Liquid, Polymeric Resins, and Supercritical CO2. ACS Sustainable Chemistry & Engineering, 4(6), 3357- 3367.
- Di Giacomo, G., Brandani, V., Del Re, G., Martınez de la Ossa, E. (1991) Selectivity and loading behavior in liquid carbon dioxide extraction of ethanol from dilute aqueous solutions. Chem. Biochem. Eng. Q., 5, 141–144.
- Díaz-Reinoso, B., Moure, A., Domínguez, H., a Parajó, J. C. (2006). Supercritical CO2 Extraction and Purification of Compounds with Antioxidant Activity. Journal of Agricultural and Food Chemistry, 54 (7), 2441-2469.
- Fersi, W., Baaka, N., Dhaouadi, H. (2024). Evaluating Supercritical Carbon Dioxide Extraction vs. Conventional Aqueous Extraction of Natural Dyes from Hypericum triquetrifolium for Textile Fibers Dyeing. Fibers Polym, 25, 3841–3851.
- Grierson, S., Strezov, V., Bray, S., Mummacari, R., Danh, L. T., Foster, N. (2012). Assessment of Bio-oil Extraction from Tetraselmis chui Microalgae Comparing Supercritical CO2, Solvent Extraction, and Thermal Processing Energy Fuels, 26(1), 248–255.
- Ivakhnov, A. D., Skrebets, T. E. (2024). Supercritical Fluid Extraction of Avermectin from Streptomyces avermitilis Using Carbon Dioxide. Russ. J. Phys. Chem. B, 18, 1737–1744.
- Ivanović, J., Đilas, S., Jadranin, M., Vajs, V., Babović, N., Petrović, S., Žižović, I. (2009). Supercritical carbon dioxide extraction of antioxidants from rosemary (Rosmarinus officinalis L.) and sage (Salvia officinalis L.). Journal Serb. Chem. Soc. 74(7), 717–732.
- Kessler, C. J., Martins, M. I., Manrique, A. Y, Rodrigues, E. A., Filomena B. M., Dias, M. M. (2024). Advancements in conventional and supercritical CO2 extraction of Moringa oleifera bioactives for cosmetic applications: A review. The Journal of Supercritical Fluids, 214, 106388.
- Kotnik, P., Skerget, M., Knez, Z. (2007). Supercritical fluid extraction of chamomile flower heads: Comparison with conventional extraction, kinetics and scale-up. Journal of Supercritical Fluids, 43, 192–198.
- Kumar, S., Rai, A. & Prasad, K. (2024). Optimization and multifactor assessments of supercritical Moringa oleifera seed oil extraction. Food Measure.
- López-Limón, J. A., Hernández-Cázares, A. S., Hidalgo-Contreras, J. V., De la Vega, G. Romero, Mellado-Pumarino, R. A., Ríos-Corripio, M. A. (2025). Effect of supercritical CO2 extraction as pretreatment to obtain C-phycocyanin from spirulina (Arthrospira maxima). The Journal Supercritical Fluids, 215, 106428.
- Mukhopadhyay M., Chapter: Fundamentals of supercritical fluids and phase equilibria in Natural Extracts Using Supercritical Carbon Dioxide, (2000) CRC Press.
- Mantell, C., Casas, L., Rodríguez, M., Martínez de la Ossa, Enrique. (2013). Supercritical Fluid Extraction, Book Editor(s): Shri Ramaswamy, Hua-Jiang Huang, Bandaru V. Ramarao.
- Min, J., Li, Shufen., H. J., Liu, N. (2010). Supercritical CO2 Extraction of Jatropha Oil and Solubility Correlation. Journal of Chemical & Engineering, 55 (9), 3755-3758.
- Mohammadi, M. A., Safavizadeh, V., Y, M. (2024.) A short review of supercritical fluid extraction of plant extracts. Food Measure, 18, 3651–3664.
- Mouahid, A., Rébufa, C., Le Dréau, Y. (2024). Supercritical CO2 extraction of Walnut (Juglans regia L.) oil: Extraction kinetics and solubility determination. The Journal of Supercritical Fluids, 211, 106313.
- Pereyra, C., Molero, A., Martınez de la Ossa, E., 1995. Extraccion supercrıtica con dioxido de carbono. Ingenierıa Quımica Junio, 181–184.
- Reverchon, E., De Marco, I. (2006). Supercritical fluid extraction and fractionation of natural matter. Journal of Supercritical Fluids, 38, 146–166.
- Romano, R., De Luca, L., Aiello, A., Rossi, D., Pizzolongo, F., Masi, P. (2022). Bioactive compounds extracted by liquid and supercritical carbon dioxide from citrus peels. International Journal of Food Science and Technology, 57, 3826–3837.
- Ruiz-Rodriguez, A., Reglero, G., Ibanez, E. (2010). Recent trends in the advanced analysis of bioactive fatty acids. Journal of Pharmaceutical and Biomedical Analisis, 51, 305–326.
- Sabio, E., Lozano, M., Montero de Espinosa, V., Mendes, R. L., Pereira, A.P., Palavra, A. F., Coelho, J.A. (2003). Lycopene and β-Carotene Extraction from Tomato Processing Waste Using Supercritical CO2 Industrial & Engineering Chemistry Research, 42(25), 6641-6646.
- Sahena, F., Zaidul, I. S., Jinap, S., Karim, A. A., Abbas, K. A., Norulaini, N. A., Omar, A. K. (2009.) Application of supercritical CO2 in lipid extraction—A review. Journal of Food Engineering, 95, 240–253.
- Saldaña, D. A. M., Mohamed, S. R., Baer, G. M., Mazzafera, P. (1999). Extraction of Purine Alkaloids from Maté (Ilex paraguariensis). Using Supercritical CO2. Journal of Agricultural and Food Chemistry, 47(9), 3804-3808.
- Sato, T., Fang, Z., Smith Jr., R.L., Xu, L. (2022). Production of Valuable Compounds from Leaves by Supercritical CO2 Extraction. Production of Biofuels and Chemicals from Sustainable Recycling of Organic Solid Waste. Biofuels and Biorefineries, 11. Springer, Singapore.
- Shi, J., Xue, S., Sun, Q., Scanlon, M. (2024). Effects of solubility of Supercritical-CO2 solvent and mass transfer property on extraction of vitamin E from canola seeds LWT, 203, 116302.
- Shi, J., Xue, S., Sun, Q. (2025.) Exploring Selectivity of Supercritical-CO2 for Vitamin E Extraction from Canola Seeds. Food Bioprocess Technol, 18, 2754–2764.
- Singh, S., Verma, D. K., Thakur, M., Tripathy, S., Patel, R. A., Shah, N., Utama, G. L., Srivastav,
- P., Roberto, J., Benavente-V., Chavez-Gonzalez, L. M., Aguilar, C. N. (2021). Supercritical fluid extraction (SCFE) as green extraction technology for high-value metabolites of algae, its potential trends in food and human health. Food Research International, 150, 110746.
- Talmaciu, A. I., Volf, I., Popa, I. V. (2015). REVIEW: A Comparative Analysis of the ÐGreenÏ Techniques Applied for Polyphenols Extraction from Bioresources, Chem Biodivers. 12(11):1635- 51.
- Temelli, F. (2009.) Perspectives on supercritical fluid processing of fats and oils. Journal of Supercritical Fluids, 47, 538–590.
- Yıldırım, M., Ersatır, M., Poyraz, S., Amangeldinova, M., Kudrina O. N., Terletskaya, V. N. (2024). Green Extraction of Plant Materials Using Supercritical CO2: Insights into Methods, Analysis, and Bioactivity, 13(16), 2295.
