7th International Scientific Conference Modern Trends in Agricultural Production, Rural Development and Environmental Protection (2025) [pp. 151-159]
AUTHOR(S) / AUTOR(I): Bardha Ibishi
, Vesna Karapetkovska-Hristova
, Nexhbedin Beadini
, Bardhyl Limani
, Ivan Pavlović 
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DOI: 10.46793/7thMTAgricult.14I
ABSTRACT / SAŽETAK:
The rising demand for organic and residue-free poultry products has increased interest in natural feed additives as alternatives to synthetic growth promoters. This study evaluated the effects of Thymus vulgaris (thyme) and raw propolis supplementation on the growth performance of broiler chickens reared under extensive conditions. A total of 40 cross-breed day-old chicks were used in a seven-week experiment conducted in a village in the Polog region. The birds were assigned to control and treatment groups, receiving either 25 g kg-1 thyme, 3 g kg-1 propolis, or 6 g kg-1 propolis in their diets through out the trial. Body weights were recorded weekly, and statistical analyses were conducted to evaluate the effects of the treatments. No significant differences in body weight were observed during the first six weeks of fattening (p > 0.05). However, in the seventh week, broilers supplemented with 6 g kg-1 propolis exhibited a statistically significant increase in body weight compared to the control and lower-dose propolis groups (p < 0.01). These findings suggest a dose- and time-dependent response, indicating that prolonged supplementation and higher inclusion levels may be necessary to achieve measurable growth benefits. The delayed effect may be related to metabolic adaptations and improved nutrient utilization influenced by the antioxidant and immunomodulatory properties of propolis and thyme. The results support the potential of propolis and thyme as natural and effective growth promoters, particularly in sustainable and regionally adapted poultry systems where antibiotic use is restricted. This study contributes to the growing body of evidence supporting phytogenic feed additives as viable alternatives to antibiotics, addressing concerns related to antimicrobial resistance and consumer preference for natural poultry products.
KEYWORDS / KLJUČNE REČI:
propolis, thymus vulgaris, broiler chickens, growth performances, polog region
ACKNOWLEDGEMENT / PROJEKAT:
REFERENCES / LITERATURA:
- Abbas, R.J., Al-Badri, R.A., & Al-Mayah, A.A. (2022). Propolis inclusion into broiler diets improves the immunomodulation and productive performance after challenge with Escherichia coli infection. Pakistan Journal of Zoology, 54(1), 1–9.
- Abd El-Latif, S.A., Ahmed, F.A., & El-Kaiaty, A.M. (2002). Effect of feeding dietary thyme, black cumin, dianthus and fennel on productive and some metabolic responses of growing Japanese quail. Egyptian Poultry Science Journal, 22(1), 109–125.
- Al-Hariri, M.T. (2011). Propolis and its direct and indirect hypoglycemic effect. Journal of Family and Community Medicine, 18(3), 152–154.
- Attia, Y.A., Al-Hanoun, A., El-Gandy, M.F., Abdella, M.M. I., El-Shafei, M., & Al-Harthi, M.A. (2017). Effects of supplementing diet with thyme (Thymus vulgaris L.) essential oil and/or selenium yeast on production performance and blood variables of broiler chickens. Poultry Science, 96(7), 1980–1989.
- Bueno-Silva, B., Marsola, A., Ikegaki, M., Alencar, S.M., & Rosalen, P.L. (2017). The effect of seasons on Brazilian red propolis and its botanical source: Chemical composition and antibacterial activity. Natural Product Research, 31(11), 1318–1324.
- Chan, G.C.F., Cheung, K.W., & Sze, D.M.Y. (2013). The immunomodulatory and anticancer properties of propolis. Clinical Reviews in Allergy & Immunology, 44, 262–273.
- Gulcin, I., Oktay, M., Kirecci, E., & Kufrevioglu, O.I. (2004). Antioxidant activity of Thymus vulgaris extracts. Journal of Agricultural and Food Chemistry, 52(16), 4831–4835.
- Gumus, R., Ercan, N., & Imik, H. (2017). The effect of thyme essential oil (Thymus vulgaris) added to quail diets on performance, some blood parameters, and antioxidative metabolism of the serum and liver tissues. Revista Brasileira de Ciência Avícola, 19(2), 297–304.
- López, B.G.C., & Sawaya, A.C.H.F. (2012). A review of the plant origins, composition and biological activity of red propolis. In Natural products: Structure, bioactivity and applications (pp. 83–96).
- Mahmoud, U.T., Abdel-Rahman, M.A., & Darwish, M.H.A. (2013). The effect of Chinese propolis supplementation on Ross broiler performance and carcass characteristics. Journal of Advanced Veterinary Research, 3(4), 154–160. https://advetresearch.com/index.php/AVR/article/view/136
- Marshall, B.M., & Levy, S.B. (2011). Food animals and antimicrobials: Impacts on human health. Clinical Microbiology Reviews, 24(4), 718–733. https://doi.org/10.1128/CMR.00002-11
- Popović, S., Puvača, N., Kostadinović, L., Džinić, N., Bošnjak, J., Vasiljević, M., et al. (2016). Effects of dietary essential oils on productive performance, blood lipid profile, enzyme activity and immunological response of broiler chickens. European Poultry Science, 80, 1–12.
- Ragaa, N.M., El-Gendi, G.M., & El-Bana, M.A. (2016). The effect of thyme oil on performance, oxidative status, and immunity of broiler chickens. Egyptian Poultry Science Journal, 36(2), 563–576.
- Rulebook on the manner of sampling and methods of analysis and super-analysis of bee products. (2011). Official Gazette of the Republic of Macedonia, No. 67/2011.
- Sadarman, S., Putra, E., & Darminto, W. (2021). Propolis supplementation affects performance, intestinal morphology, and bacterial population of broiler chickens. South African Journal of Animal Science, 51(4).
- Saleh, N., Allam, T., El-Latif, A.A., & Ghazy, E. (2014). The effects of dietary supplementation of different levels of thyme (Thymus vulgaris) and ginger (Zingiber officinale) essential oils on performance, hematological, biochemical and immunological parameters of broiler chickens. Global Veterinaria, 12(6), 736–744.
- Sforcin, J.M. (2016). Biological properties and therapeutic applications of propolis. Phytotherapy Research, 30, 894–905.
- Shaddel-Tili, A., Eshratkhan, B., Kouzehgari, H., & Ghasemi-Sadabadi, M. (2017). The effect of different levels of propolis in diets on performance, gastrointestinal morphology and some blood parameters in broiler chickens. Bulgarian Journal of Veterinary Medicine, 20(3), 215–224.
- Tolba, A.A.H., & Hassan, M.S.H. (2003). Using some natural additives to improve physiological and productive performance of broiler chicks under high temperature conditions. 2-Black cumin (Nigella sativa) or garlic (Allium sativum). Egyptian Poultry Science Journal, 23, 327–340.
- Toukala, J.P., Tadondjou, C.D.A., Ojong, T.E., Feugaing, D.D.S., Sylviane, M., Vemo, N.B., & Ngoula, F. (2020). Effects of feed supplementation of Thymus vulgaris (Lamiaceae) powder on growth performance, carcass characteristics and blood profiles of maturing Cameroonian local kabir rooster. International Journal of Agronomy and Agricultural Research, 17(4), 1–10.