Occurrence of Rubus yellow net virus in red raspberry orchards in Serbia

7th International Scientific Conference Modern Trends in Agricultural Production, Rural Development and Environmental Protection (2025) [pp. 91-95]  

AUTHOR(S) / АУТОР(И): Bojana Vasilijević , Darko Jevremović

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DOI: 10.46793/7thMTAgricult.08V

ABSTRACT / САЖЕТАК:

A survey for Rubus yellow net virus (RYNV) in red raspberry orchards in Serbia was conducted from 2014‒2022. A total of 112 samples from seven cultivars were collected and analyzed. Reverse transcription polymerase chain reaction (RT-PCR) using RYNV-specific primers was used for virus detection. A specific 350 bp fragment was obtained in 30 samples, confirming RYNV presence in cultivars ‘Fertödi Zamatos’, ‘Meeker’, ‘Tulameen’, and ‘Willamette’. Sequence analysis of the partial polyprotein gene of the RYNV genome confirmed that selected Serbian isolates share 83.43–98.29% nucleotide identities with RYNV sequences in GenBank.

KEYWORDS / КЉУЧНЕ РЕЧИ:

red raspberry, RYNV, RT-PCR, phylogenetics

ACKNOWLEDGEMENT / ПРОЈЕКАТ:

This study was supported by the Ministry of Technological Development and Innovations of the Republic of Serbia, Contract No. 451-03-136/2025-03/200215.

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