8th WORKSHOP: FOOD AND DRUG SAFETY AND QUALITY, (pp.143–146)
АУТОР / AUTHOR(S): A. Ignjatijević 
, S. Vuletić , B. Nikolić , D. Mitić-Ćulafić , T. Ganić , T. Anđić , S. Cvetković Šobot
DOI: 10.46793/8FDSQ.PC3AI
САЖЕТАК / ABSTRACT:
Genotoxicity refers to the ability of various agents, called genotoxins, to induce damage to genetic material. Given their involvement in various human diseases, exploring new protective compounds from natural sources, especially plants, is of crucial importance today. With that in mind, our study evaluated the potential antigenotoxic effect of methanolic and dichloromethane extracts of traditionally used plant Achillea millefolium on normal fetal fibroblasts (MRC-5). The screening of cytotoxicity was conducted using an MTT assay to determine non-cytotoxic concentrations of extracts on MRC-5 cells. Genotoxicity and antigenotoxicity were assessed using an alkaline comet assay. Non-genotoxic concentrations were determined for antigenotoxicity testing, which showed that extract AmDC and lower concentrations of AmM exhibited significant protective effects. The results of this study strongly encourage further investigations of the protective properties of A. millefolium.
КЉУЧНЕ РЕЧИ / KEYWORDS:
ПРОЈЕКАТ / ACKNOWLEDGEMENT:
We thank Dr. Jelena Kukić-Marković, University of Belgrade – Faculty of Pharmacy, for the preparation of the extract. Supported by the Ministry of Science, Technological Development and Innovation of the Republic of Serbia (Contract No. 451-03-66/2024-03/200178 and No. 451-03-65/2024-03/ 200178).
ЛИТЕРАТУРА / REFERENCES:
[1] A. Hartwig, M. Arand, B. Epe, S. Guth, G. Jahnke, A. Lampen, H. Martus, B. Monien, I. M. C. M. Rietjens, S. Schmitz-Spanke, G. Schriever-Schwemmer, P. Steinberg, G. Eisenbrand, Arch. Toxicol., 2020, 94, 1787–1877.
[2] S.T. Asma, U. Acaroz, K. Imre, A. Morar, S.R.A. Shah, S.Z. Hussain, D. Arslan-Acaroz, H. Demirbas, Z. Hajrulai-Musliu, F.R. Istanbullugil, A. Soleimanzadeh, D. Morozov, K. Zhu, V. Herman, A. Ayad, C. Athanassiou, S. Ince, Cancers, 2022, 14, 6203.
[3] I. Süntar, Phytochem. Rev., 2020, 19, 1199-1209.
[4] B. F. Far, G. Behzad, H. Khalili, Heliyon, 2023, 9, e22841.
[5] M. Akram, J. Membr. Biol., 2013, 246, 661-663.
[6] D. Kanojia, M.M. Vaidya, Oral Oncol., 2006, 42, 655-667.
[7] S. Cvetković, B. Nastasijević, D. Mitić-Ćulafić, S. Đukanović, D. Tenji, J. Knežević-Vukčević, B. Nikolić, Mutat. Res. Genet. Toxicol. Environ. Mutagen., 2020, 858-860, 503251.
[8] E. J. Calabrese, M. P. Mattson, NPJ Aging Mech. Dis., 2017, 3, 1–8.
[9] G. G. Toplan, T. Taşkın, G. İşcan, F. Göger, M. Kürkçüoğlu, A. Civaş, G. Ecevit-Genç, A. Mat, K. H. C. Başer, Molecules, 2022, 27, 1956.
[10] Luca, V. S., Miron, A., & Aprotosoaie, A. C.), Phytochemistry Reviews, 2016, 15(4), 591–625.