2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023 (507-510)
АУТОР(И) / AUTHOR(S): Danijela Stojković, Maja Đukić, Marija Ristić, Marina Ćendić Serafinović, Svetlana Belošević, Emina Mrkalić, Ivan Jakovljević
Е-АДРЕСА / E-MAIL: danijela.stojkovic@kg.ac.rs
DOI: 10.46793/ICCBI23.507S
САЖЕТАК / ABSTRACT:
The synthesis and characterization of a newly synthesized piano-stool [Ru-(ɳ6-p-cymene)Cl2L] complex with a ligand 3-amino-2-cyano-N-phenyl-3-(4-phenyl-1-piperazinyl)-2-propenethioamide are presented. The complex was obtained in good yield as an ochre powderand was characterized by elemental analysis, 1H and 13C NMR, IR, conductometry, and melting point. The fluorescence binding studies showed that the interaction of the complex with albumin occurs by a static quenching mechanism and that the complex showed a very high value of the binding constant with one binding site (Kb = 1.68 × 107; n = 1.37). In order to identify the binding location in the HSA molecule, competitive experiments with site markers (eosin Y (site I) and ibuprofen (site II)) were performed. Obtained results showed that the examined complex binds to the site I of subdomain IIA (for eosin Y: Kb = 5.94 × 102; n = 0.65 vs. for ibuprofen: Kb = 1.38 × 107; n= 1.34).
КЉУЧНЕ РЕЧИ / KEYWORDS:
ruthenium(II) complex, thioamide, synthesis, HSA interactions
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