2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023 (415-418)
АУТОР(И) / AUTHOR(S): Mirjana B. Čolović, Jelena J. Žakula, Lela B. Korićanac, Nada Savić, Tatjana Parac-Vogt, Danijela Z. Krstić
Е-АДРЕСА / E-MAIL: colovicm@vin.bg.ac.rs
DOI: 10.46793/ICCBI23.415C
САЖЕТАК / ABSTRACT:
The aim of this study was to assess in vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster, α2-K10P2W17O61.20H2O (lacunary WD) against cervical carcinoma HeLa cells as a commonly used model system for the evaluation of antitumor properties. After HeLa cells had been exposed to the investigated polyoxotungstate (the concentration range of 0.001 – 1 mM) for 24, 48, and 72 h, relative cell viability (expressed as a percentage of control) was determined. The obtained results showed that lacunary WD affected HeLa cell viability in a concentration-and time-dependent manner. IC50 values (in μM), calculated using sigmoidal fitting experimental plots, were as follows: 24.11 ± 9.95, 12.74 ± 0.096, and 11.48 ± 0.12 for 24, 48, and 72 hours treatment, respectively. In comparison with cisplatin, (positive control), IC50 values (μM) for 24 hours treatment were similar – 24.11 (lacunary WD) vs. 24.49 (cisplatin). However, after 48 and 72 hours IC50 obtained for cisplatin were found to be lower – 8.81 and 4.93 μM, respectively.
Accordingly, the studied WD polyoxotungstate could not be regarded as a superior anticancer agent in comparison with the standard chemotherapeutic. Nevertheless, this studied nanocluster deserves attention as a promising antitumor therapeutic and as a good platform for the design of next-generation metal-based anticancer agents.
КЉУЧНЕ РЕЧИ / KEYWORDS:
antitumor metallodrug, HeLa cells, in vitro cytotoxicity, monolacunary Wells-Dawson, polyoxotungstate
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