The antioxidant capacity determination of Rosa canina L. fruit extracts using optimized cyclic voltammetry and spectrophotometric assays, and determination of vitamin C

Chemia Naissensis Volume 7, No.2 (2025) (стр. 36-5)   

АУТОР(И) / AUTHOR(S): Katarina Milenković , Jelena Mrmošanin , Dalibor Stanković , Dobrila Ranđelović , Stefan Petrović , Denis Mitov , Aleksandra Pavlović

 

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DOI: 10.46793/ChemN7.2.36M

САЖЕТАК / ABSTRACT:

The optimized electrochemical method, cyclic voltammetry, was applied to investigate the antioxidant capacity of water extracts of Rosa canina L. fruits. Scan speeds (25, 50, and 75 mV/s) and pH (2, 4.5, and 7) were varied and optimal conditions were found at 75 mV/s and pH 4.5. Cyclic voltammograms, recorded from 0 to 1200 mV, showed anodic peaks for catechin-type flavonoids and quercetin. Spectrophotometric tests (FRAP, CUPRAC, DPPH, ABTS) and determination of total polyphenols content (TPC), total flavonoids content (TFC), and vitamin C content were also conducted. FRAP and CUPRAC values ranged from 0.534 to 0.710 mmol Fe/g dw and from 103 to 174 mg TE/g dw, respectively. ABTS values were in the interval from 566 to 623 mg TE/g dw, while DPPH values ranged from 113 to 188 mg TE/g dw. Total polyphenols content and total flavonoids content ranged from 24.0 to 38.9 mg GAE/g dw, and from 12.7 to 21.5 mg CE/g dw, respectively. The concentrations of vitamin C were in the interval from 2.78 to 3.67 mg/g dw. Pearson’s analysis showed good correlations between ABTS and DPPH (R² = 0.87), FRAP and DPPH (R² = 0.85), FRAP and ABTS (R² = 0.86), TPC and in vitro tests (0.5 < R² < 0.77), and TFC and in vitro tests (0.47 < R² < 1). For the purpose of classifying spectrophotometric tests for determining antioxidant activity, hierarchical cluster analysis was performed.

КЉУЧНЕ РЕЧИ / KEYWORDS:

Rosa canina L., antioxidant activity, cyclic voltammetry, spectrophotometric assays, cluster analysis, Pearson correlation analysis

ПРОЈЕКАТ/ ACKNOWLEDGEMENT:

This research was supported by the Ministry of Science, Technological Development, and Innovation of Republic of Serbia (Contracts No. 451-03-47/2023-01/200124, 451-03-65/2024- 03/200124,  451-03-66/2024-03/200124,  451-03-136/2025-03/200124  and  451-03-137/2025-03/200124).

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