MICROBIAL CONVERSION OF NON-FOOD FEEDSTOCK INTO VALUE-ADDED BIOPRODUCTS

Replacing food-based feedstocks with non-food alternatives is crucial for food security and the sustainable supply of raw materials for biomanufacturing. In this article, advanced technologies for microbial strain improvement are summarized. Then examples on the valorization of non-food biomass by engineered microbial strains are reviewed, including the development of integrated processing for ethanol-biogas-biofertilizer coproduction, green biomanufacturing of lactic acid, and the levan production using sucrose-rich industrial wastewater.

KEYWORDS / KLJUČNE REČI: 

Non-food biomass, biomanufacturing, microbial strain improvement, metabolic engineering, synthetic biology

ACKNOWLEDGEMENT / PROJEKAT:

REFERENCES / LITERATURA:

• Bo Wu, Han Qin, Yi-Wei Yang, Guo-Wei Duan, Chun-Yan Zhao, Fu-Rong Tan, Feng-Jiao Cao, Guo-Quan Hu and Ming-Xiong He (2019). Engineered Zymomonas mobilis tolerant to acetic acid and low pH via multiplex atmospheric and room temperature plasma (mARTP). Biotechnology for Biofuels, doi: 1186/s13068-018-1348-9
• Brenac, L., Baidoo, E. E. K., Keasling, J. D., & Budin, I. (2019). Distinct functional roles for hopanoid composition in the chemical tolerance of Zymomonas mobilis. Molecular Microbiology, doi: 10.1111/mmi.14380
• China Statistical www.stats.gov.cn/english/
• Duan, G., Zhou, Y., Qin, Y., Gong, G., Wu, B., Li, J., Zhuang, Y., He, M. (2019). Replacing water and nutrients for ethanol production by ARTP-derived biogas slurry tolerant Zymomonas mobilis strain. Biotechnology for Biofuels, doi: 10.1186/s13068-019-1463-2
• Jing-Li Wang, Bo Wu, Han Qin, Yang You, Song Liu, Zong-Xia Shui, Fu-Rong Tan, Yan-Wei Wang, Qi-Li Zhu, Yan-Bin Li, Zhi-Yong Ruan, Ke-Dong Ma, Li-Chun Dai, Guo-Quan Hu & Ming-Xiong He (2016). Engineered Zymomonas mobilis for salt tolerance using EZ-Tn5-based transposon insertion mutagenesis system. Microbial Cell Factories, doi: 10.1186/ s12934-016-0503-x
• Lin-Ping Yu, Fu-Qing Wu, Guo-Qiang Chen (2019), Next-Generation Industrial Biotechnology-Transforming the Current Industrial Biotechnology into Competitive Processes. Biotechnology Journal, https://doi.org/10.1002/ 201800437
• Liu, L., Zhou, Z., Gong, G., Wu, B., Todhanakasem, T., Li, J., Zhuang, Y., & He, M. (2024). Economic co-production of cellulosic ethanol and microalgal biomass through efficient fixation of fermentation carbon dioxide. Bioresource Technology, doi: 10.1016/j.biortech.2024.130420
• Qinghui Yu, Yuncheng Li, Bo Wu, Wei Hu, Mingxiong He, Guoquan Hu (2020). Novel mutagenesis and screening technologies for food microorganisms: advances and prospects, Applied Microbiology and Biotechnology, doi: 1007/s00253-019-10341-z
• Tan, F., Wu, B., Dai, L., Qin, H., Shui, Z., Wang, J., Zhu, Q., Hu, G., & He,
• (2016). Using global transcription machinery engineering (gTME) to improve ethanol tolerance of Zymomonas mobilis. Microbial Cell Factories, doi: 10.1186/s12934-015-0398-y
• The Bioeconomy to 2030: designing a policy agenda, OECD report, https:// org/10.1787/9789264056886-en
• Weiting Wang, Bo Wu, Han Qin, Panting Liu, Yao Qin, Guowei Duan, Guoquan Hu, Mingxiong He (2019). Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors, doi: 1186/s13068-019-1631-4
• Xue, Z., Zhang, X. F., Li, H. P., Wang, L. Y., Zhang, C., Xing, X. H., & Bao, Y. (2014). Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool. Applied Microbiology and Biotechnology, doi: 10.1007/s00253-014-5755-y
• Yuhuan Huang, Xiaojie Wang, Mao Chen, Yanran Wu, Tatsaporn Todhanakasem, Nan Peng, Mingxiong He, Bo Wu (2025). Engineering Zymomonas mobilis for improving genetic transformation and stability of multi-gene biosynthetic pathways. Bioresource Technology, https://doi.org/10.1016/j.biortech.2025.133198
• Zong-Xia Shui 1, Han Qin, Bo Wu, Zhi-yong Ruan, Lu-shang Wang, Fu-Rong Tan, Jing-Li Wang, Xiao-Yu Tang, Li-Chun Dai, Guo-Quan Hu, Ming-Xiong He (2015). Adaptive laboratory evolution of ethanologenic Zymomonas mobilis strain tolerant to furfural and acetic acid Applied Microbiology and Biotechnology, doi: 10.1007/s00253-015-6616-z