AUTHOR(S) / АУТОР(И): Srđan Tufegdžić 
, Marijana Živković
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DOI: https://doi.org/10.46793/SBT26.873T
ABSTRACT / САЖЕТАК:
An enzyme-assisted green approach was developed for Hantzsch and Biginelli multicomponent reactions using benzaldehyde, acetylacetone, and urea in a glycerol–water buffer. Two known compounds were prepared using identical temperature profiles, citrate buffers, MgSO₄, and glycerol as an emulsion stabilizer. Product chemoselectivity was controlled by tuning pH, reagent ratios, MgSO₄ concentration, and the presence or absence of urease. The results demonstrate biotechnology-assisted pathway control in competing multicomponent reactions within a sustainable synthetic framework. Reactions at 65 °C afforded ~40% yields, with fluorescence properties confirming product formation.
KEYWORDS / КЉУЧНЕ РЕЧИ:
green chemistry, heterocyclic chemistry, Biginelli reaction, Hantzsch reaction, urease (Canavalia ensiformis)
ACKNOWLEDGEMENT / ПРОЈЕКАТ:
The research presented in this article was financially supported by the Ministry for Science, Technological Development and Innovation of the Republic of Serbia (Grant no. 451-03-136/2025-03/200026)
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