IN VITRO PERFORMANCE, PHYSIOLOGICAL AND BIOCHEMICAL TRAITS OF PPV-INFECTED AND VIRUS-FREE ‘CRVENA RANKA’

4th International Symposium On Biotechnology (2026),  [pp. 247-255]
 
AUTHOR(S) / АУТОР(И): Tatjana Anđelić, Mirjana Mićević, Tatjana Vujović, Bojana Vasilijević, Boris Rilak , Aleksandra Kalezić-Glišović  

 

Download Full Pdf   

DOI: https://doi.org/10.46793/SBT26.247A

ABSTRACT / САЖЕТАК:

The specific growth and metabolic conditions in in vitro culture prevent visible symptoms of Plum pox virus infection, while the virus persists in the plant. We assessed whether stress-related parameters, including pigment content and hydrogen peroxide levels, are altered under these conditions. Differences in the establishment and maintenance of tissue cultures derived from infected and virus-free plants were also examined. Our results show that viral infection affects metabolism and stress responses under in vitro conditions, highlighting the importance of physiological status and genotype in micropropagation strategies

KEYWORDS / КЉУЧНЕ РЕЧИ:

Plum pox virus, ‘Crvena Ranka’, micropropagation, leaf pigments, plant stress

ACKNOWLEDGEMENT / ПРОЈЕКАТ:

This work was supported by the Ministry of Science, Technological Development, and Innovation of the Republic of Serbia under Grant numbers 451-03-33/2026-03/200215 and 451-03-33/2026-03/200132.

REFERENCES / ЛИТЕРАТУРA:

  • Abdelwahab Elansary, D.W., Gürcan, K., Roumi, V., and Şimşek, Ö. (2024). Efficacy of Plant Tissue Culture Techniques for Eliminating Black Mulberry Idaeovirus (BMIV) from Infected Black Mulberry (Morus nigra). Plants. 13(21): 2959.
  • Alexieva, V., Sergiev, I., Mapelli, S., and Karanov, E. (2001). The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant, Cell & Environment. 24(12): 1337–1344.
  • Cheaib, A., and Killiny, N. (2024). Photosynthesis Responses to the Infection with Plant Pathogens. Molecular Plant-Microbe Interactions. 38(1): 9–29.
  • Clemente-Moreno, M.J., Díaz-Vivancos, P., Rubio, M., Fernández-García, N., and Hernández, J.A. (2013). Chloroplast protection in plum pox virus-infected peach plants by L-2-oxo-4-thiazolidine-carboxylic acid treatments: effect in the proteome. Plant, Cell & Environment. 36(3): 640–654.
  • Clemente-Moreno, M.J., Hernández, J.A., and Diaz-Vivancos, P. (2015). Sharka: how do plants respond to Plum pox virus infection? Journal of Experimental Botany. 66(1): 25–35.
  • Díaz-Vivancos, P., Clemente-Moreno, M.J., Rubio, M., Olmos, E., García, J.A., Martínez-Gómez, P., and Hernández, J.A. (2008). Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus. Journal of Experimental Botany. 59(8): 2147–2160.
  • García, J.A., Rodamilans, B., Martínez-Turiño, S., Valli, A.A., Simón-Mateo, C., and Cambra, M. (2025). Plum pox virus: An overview of the potyvirus behind sharka, a harmful stone fruit disease. Annals of Applied Biology. 186(1): 49–75.
  • Glišić, I.S., Paunović, G., Glišić, I.P, Milošević, N., and Popović, B. (2018). The production and properties of some autochthonous plum cultivars suitable for brandy production in Serbia. Journal of Mountain Agriculture on the Balkans. 21 (4): 192–206.
  • Jevremović, D., Vujović, T., Milošević, N., and Paunović, S.A. (2021). Health status assessment of the Serbian autochthonous plum cultivars for cryopreservation purposes. Acta Horticulturae. 1322: 77–82.
  • Lichtenthaler, H.K. (1987). Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Published in Methods in Enzymology, vol. 148, Douce R., Packer L. (eds), pp 350-382. New York, USA: Academic Press.
  • Milosevic, T., and Milosevic, N. (2012). Phenotypic diversity of autochthonous European (Prunus domestica L.) and Damson (Prunus insititia L.) plum accessions based on multivariate analysis. Horticultural Science. 39(1): 8–20.
  • Mihaljević, I., Dugalić, K., Tomaš, V., Viljevac, M., Pranjić, A., Čmelik, Z., Puškar, B., Jurković, Z. (2013). In vitro sterilization procedures for micropropagation of ‘oblačinska’ sour cherry. Journal of Agricultural Sciences. 58(2):117–126.
  • Murashige, T., and Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum. 15(3), 473-497.
  • Pedrelli, A., Ricci, G.P., Panattoni, A., Nali, C., and Cotrozzi, L. (2023). Physiological and Biochemical Responses Induced by Plum Pox Virus and Plum Bark Necrosis Steam Pitting Associated Virus in Tuscany Autochthonous Plum cv. Coscia di Monaca. Plants. 12(18): 3264.
  • Pérez-Caselles, C., Alburquerque, N., Martín-Valmaseda, M., Alfosea-Simón, F.J., Faize, L., Bogdanchikova, N., et al. (2025). Nanobiotechnology for efficient plum pox virus elimination from apricot plants. Plant Science. 352: 112358.
  • Popović, B., Nikićević, N., Tešević, V., Urošević, I., Mitrović, O., Kandić, M. (2015). Sensory properties of plum brandies obtained by blending distillates of plum cultivar ‘Crvena Ranka’ and other cultivars. Voćarstvo. 49(191-192): 99–105.
  • Saeedi, S.A., Vahdati, K., Sarikhani, S., Daylami, S.D., Davarzani, M., Gruda, N.S., and Aliniaeifard, S. (2023). Growth, photosynthetic function, and stomatal characteristics of Persian walnut explants in vitro under different light spectra. Frontiers in Plant Science. 14: 1292045.
  • Vujović, T., Jevremović, D., Marjanović, T., and Glišić, I. (2020). In vitro propagation and medium-term conservation of autochthonous plum cultivar ‘Crvena Ranka’. Acta agriculturae Serbica. 25(50): 141–147.
  • Vujović, T., Jevremović, D., Glišić, I. S., Milošević, N., and Anđelić, T. (2021). In vitro culture establishment and shoot multiplication of eight autochthonous plum genotypes. Acta Horticulturae. 1322: 179–186.