ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF ZIZYPHUS JUJUBA L. LEAF EXTRACTS

2nd International Symposium On Biotechnology (2024),  [415-422]

AUTHOR(S) / АУТОР(И): Vesna Đurović, Sofija Radenković, Leka Mandić, Mirjana Radovanović, Marijana Pešaković, Ivana Bošković, Dragutin Đukić

E-MAIL: vesnadjurovic@kg.af.rs

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DOI: 10.46793/SBT29.53VDJ

ABSTRACT / САЖЕТАК:

The aim of the present study was to investigate the biological activities of the extracts obtained from leaf of Ziziphus jujuba. To reach this purpose, total phenolic content (TPC), total flavonoid content (TFC), DPPH and ABTS radical scavenging activity, as well as antibacterial activity extracts were evaluated. The content of total phenols in leaf ranged from 22.71 to 28.69 mg GAE/g, the total content of flavonoids ranged from 5.15 mg RE/g to 8.25 mg RE/g depending on the applied extraction method. All extracts showed very high antioxidant activity. The ethanol extracts of Ziziphus jujuba leaf were screened for antibacterial activities against Salmonella typhimurium, Salmonella enteritidis, Listeria ivanovii, Listeria monocytogenes, Staphylococcus aureus and Pseudomonas aerogenes using microdilution method. Furthermore, the highest antibacterial activity was observed against Salmonella typhimurium and Salmonella enteritidis. The present results suggested the promising antioxidant and antibacterial properties of Ziziphus jujuba leaf extracts, which can be used in the fabrication of functional bioactive ingredients for different purposes.

KEYWORDS / КЉУЧНЕ РЕЧИ:

phytochemicals, antibacterial evaluation, Ziziphus jujuba

REFERENCES / ЛИТЕРАТУРА:

  • Đurović V., Mandić L., Igrošanac M., Radovanović M., Pešaković M., Mladenović J., Đukić D. (2023). Celery (Apium graveolens L.) As a source of phytochemicals with antioxidant and Antibacterial effects.1st International Symposium on Biotechnology, 17–18 March 2023. University of Kragujevac, Faculty of Agronomy Čačak. Proceedings p. 315-322.
  • Esteki T., Urooj A. (2012). Antioxidant components and activity in the peel of Ziziphus jujuba Mill. Journal of Pharmaceutical Research International 5: 2705–2709.
  • Ghazghazi H., Aouadhi C., Riahi L., Maaroufi A., & Hasnaoui, B. (2014). Fatty acids composition of Tunisian Ziziphus lotus L. (Desf.) fruits and variation in biological activities between leaf and fruit extracts. Natural Product Research, 28(14), 1106–1110.
  • Hossain A., Hamood A., Humaid MT. (2016). Comparative evaluation of total phenols, flavonoids content and antioxidant potential of leaf and fruit extracts of Omani Ziziphus jujuba L. 2016; Pacific Science Review A: Nat. Sci. Eng. 18: 78–83.
  • Kavak D.D., Altıok E., Bayraktar O., & Ülkü S. (2010). Pistacia terebinthus extract: As a potential antioxidant, antimicrobial and possible β-glucuronidase inhibitor. Journal of Molecular Catalysis B: Enzymatic, 64(3), 167–171. 10.1016/j.molcatb.2010.01.029
  • Kchaou W., Abbès F., Blecker C., Attia H., Besbes S. (2013). Effects of extraction solvents on phenolic contents and antioxidant activities of Tunisian date varieties (Phoenix dactylifera L.) Industrial Crops and Products, 45 (2013), pp. 262-269.
  • Li JW., Fan L.P., Ding SD., Ding XL. (2007). Nutritional composition of five cultivars of Chinese jujube, Food Chem. 103 (2007) 454–460,
  • Ngo T.V., Scarlett C.J., Bowyer M.C., Ngo P.D., Vuong Q.V. (2017). Impact of different extraction solvents on bioactive compounds and antioxidant capacity from the root of Salacia chinensis L. Journal of Food Quality, 2017 (2017), pp. 1-8.
  • Ozturk B., Yildiz M., Yildiz K., & Gun S. (2021). Maintaining the postharvest quality and bioactive compounds of jujube (Ziziphus jujuba Mill. cv. ‘Li’) fruit by applying 1-methylcyclopropene. Scientia Horticulturae, 275, 109671.
  • Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Evans CR. (1999): Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26, 1231–1237, doi:10.1016/s0891-5849(98)00315-3.
  • Sampath Kumara KK., Chethan J., Manasa N., Ashadevi J.S., Bioactivepotential of herbaceous Phyllanthus species, Int. J. Pharm. Pharm. Sci. 4(2012) 457–461.
  • Singleton VL., Rossi JA. (1965): Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3), 144–158.
  • Tatari M., Ghasemi A., & Mousavi A. (2016). Genetic diversity in jujube germplasm (Ziziphus jujuba Mill.) based on morphological and pomological traits in Isfahan province, Iran. Crop Breeding Journal, 6(2), 79–85.
  • Wang M., Gao Q.H., Shen J., Wang X.Q., Ji X.L. (2016). The jujube (Ziziphus jujuba Mill.) fruit: A review of current knowledge of fruit composition and health benefits. J. Agric. Food Chem. 2016, 61, 3351–3363.
  • Wink M. (2008). Plant secondary metabolism: diversity, function and its evolution. 2008. Nat. Prod. Commun. 3: 1205–16. https://doi.org/10.1177/1934578X0800300801.
  • Zhang L., Liu P., Li L., HuangY., Pu Y., Hou X., Song, L. (2019). Identification and Antioxidant Activity of Flavonoids Extracted from Xinjiang Jujube (Ziziphus jujuba Mill.) Leaves with Ultra-High Pressure Extraction Technology. Molecules 24, 122. https://doi.org/10.3390/molecules24010122.