POSSIBILITY OF USING EGG SHELL AS A FLOCCULANT TO HARVEST BIOMASS OF Bacillus sp. FOLLOWING BIOTECHNOLOGICAL TREATMENT OF FOOD SECTOR WASTEWATERS

2nd International Symposium On Biotechnology (2024),  [269-276]

AUTHOR(S) / АУТОР(И): Selena Dmitrović, Ivana Danilov, Vanja Vlajkov, Tatjana Dujković, Jovana Grahovac, Aleksandar Jokić, Nataša Lukić

E-MAIL: selena.dmitrovic@uns.ac.rs

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DOI: 10.46793/SBT29.35SD

ABSTRACT / САЖЕТАК:

The aim of this study was to asses the ability of egg shell flocculation to recover bacterial Bacillus sp. BioSol021 biomass from the broth after biological treatment of wastewaters from the dairy, wine and fruit juice industries. Different concentrations of flocculant were used to estimate the effect of egg shell dosage on flocculation efficiency and antimicrobial activity against Aspergillus flavus, i.e., inhibition zone diameter. The maximum values for flocculation efficiency and inhibition zone diameter (antimicrobial activity against Aspergillus flavus) for cheese whey effluent were 94.02%, 45 mm, respectively. The maximum values, in the case of winery flotation effluent were 87.77%, 33 mm, and for the fruit juice wastwater 87.25%, 58 mm, respectively. These results indicate that egg shells could be successfully applied as the flocculating agent for separation of Bacillus sp. BioSol021 from the cultivation broth.

KEYWORDS / КЉУЧНЕ РЕЧИ:

food industry wastewaters, microbial biomass, egg shell, flocculation

REFERENCES / ЛИТЕРАТУРА:

  • Amor C., Lucas M.S., Pirra A.J., Peres J.A. (2012). Treatment of concentrated fruit juice wastewater by the combination of biological and chemical processes. Journal of Environmental Science and Health, Part A 47, 1809-1817.
  • Choi H.J. (2015). Effect of eggshells for the harvesting of microalgae species. Agriculture and Environmental Biotechnology 29, 666-672.
  • Czaja K., Góralczyk K., Struciński P., Hernik A., Korcz W., Minorczyk M., Łyczewska M., Ludwicki J.K. (2015). Biopesticides-towards increased consumer safety in the European Union. Pest Management Science 71, 3–6.
  • Damalas C.A., Koutroubas S.D. (2018). Current status and recent developments in biopesticide use. Agriculture 8, 13.
  • Dmitrović S., Lukić N., Pajčin I., Vlajkov V., Grahovac J., Jokić A. (2023). The use of chitosan for flocculation recovery of Bacillus biomass grown on dairy and wine industry effluents. Proccesses 11, 1099.
  • Dmitrović S., Pajčin I., Vlajkov V., Grahovac M., Jokić A., Grahovac J. (2022). Dairy and wine industry effluents as alternative media for the production of Bacillus-based biocontrol agents. Bioengineering 9, 663.
  • Hadiyanto H., Christwardana M., Widayat W., Jati A.K., Laes S.I. (2021). Optimization of flocculation efficiency and settling time using chitosan and eggshell as bio-flocculant in Chlorella pyrenoidosa harvesting process. Environmental Technology & Innovation 24, 101959.
  • Ioannou L.A., Puma G.L., Fatta-Kassinos D. (2015). Treatment of winery wastewater by physicochemical, biological and advanced processes: A review. Journal of Hazardous Materials 286, 343-368.
  • Qasim W., Mane A.V. (2013). Characterization and treatment of selected food industrial effluents by coagulation and adsorption techniques. Water Resources and Industry 4, 1-12.
  • Rashid N., Abdelnabi M.N.A., Vincent A.S., Mackey H.R. (2023). Simultaneous treatment of fruit juice industry wastewater and single-cell protein synthesis using purple non-sulfur bacteria. Biomas Conversion and Biorefinery 13, 16321-16332.
  • Sar T., Harirchi S., Ramezani M., Bulkan G., Yesilcimen Akbas M., Pandey A., Taherzadeh M.J. (2022). Potential utilization of dairy industries by-products and wastes through microbial processes: A critical review. Science of The Total Environment 810, 152253.
  • Sporleder M., Lacey L.A. (2013). Biopesticides. Insect Pests of Potato 463-497.
    Usmani Z., Sharma M., Gaffey J., Sharma M., Dewhurst R.J., Moreau B., Newbold J., Clark W., Kumar Thakur V., Kumar Gupta V. (2022). Valorization of dairy waste and by-products through microbial bioprocesses. Bioresource Technology 346, 126444.
  • Vlajkov V., Grahovac M., Budakov D., Loc M., Pajčin I., Milić D., Novaković T., Grahovac J. (2021). Distribution, genetic diversity and biocontrol of aflatoxigenic Aspergillus flavus in Serbian maize fields. Toxins 13, 687.
  • Yang Z., Degorce-Dumas J.R., Yang H., Guibal E., Li A., Cheng R. (2014). Flocculation of Escherichia coli using a quaternary ammonium salt grafted carboxymethyl chitosan flocculant. Environmental Science and Technology 48, 6867–6873.