1st International Symposium On Biotechnology (2023),  [39-44]

AUTHOR(S) / АУТОР(И): Jordan Marković, Đorđe Lazarević, Vladimir Zornić


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DOI: 10.46793/SBT28.039M


The aim of this study was to examine the influence of harvest time and nitrogen fertilizer application on the content of total polyphenols, flavonoids and condensed tannins in the dry matter of Italian ryegrass. The plants were harvest in 2017, in spring and early summer. The results obtained in this study showed that the contents of polyphenols, flavonoids and condensed tannins in the dry matter of Italian ryegrass (12.45 g / 100 g dry matter, 7.45 g / 100 g dry matter and 956.55 mg / 100 g dry matter, respectively) were higher when the plants harvested in early summer than in the spring. The application of nitrogen fertilizer did not have effect on the synthesis of polyphenolic compounds.


Italian ryegrass, polyphenols, flavonoids, condensed tannins


  • Agnieszka G., Grazyna A. C. (2021). Italian ryegrass (Lolium multiflorum Lam.) fiber fraction content and dry matter digestibility following biostimulant application against the background of varied nitrogen regime. Agronomy 11, 39-50.
  • Choi K. C., Son Y. O., Hwang J. M., Kim B. T., Chae M., Lee J. C. (2017). Antioxidant, anti-inflamatory and anti-septic potential of phenolic acids and flavonoids fractions isolated from Lolium multiflorum. Pharmaceutical biology 55 (1), 611-619.
  • Cosgrove G. P., Taylor P. S., Jonker A. (2015). Streep performance on perennial ryegrass cultivars differing in concentration of water-soluble carbohydrate. Journal of New Zealand Grasslands 77, 123-130.
  • Di Mola I., Ottaiano L., Cozzolino E., Senatore M., Giordano M., El-Nakhel C., Sacco A., Rouphael Y., Call G., Mori M. (2019). Plant-based biostimulants influence the agronomical, physiological and qualitative responses of baby Rocket leaves under diverse nitrogen conditions. Plants 8 (11), 522-536.
  • Đorđević N., Dinić B. (2007). Hrana za životinje. Univerzitet u Beogradu, Poljoprivredni fakultet, Beograd, Srbija.
  • Jia Z., Tang T., Wu J. (1999). The determination of flavonoids content in mulberry scavenging effect on superoxide radicals. Food Chemistry 64, 555-559.
  • Julier B., Guines F., Emile J. C., Huyghe C. (2003). Variation in protein degradability in dried forage legumes. Animal Research 52, 401-412.
  • Kagan A. I. (2021). Soluble phenolic compounds of perennial ryegrass (Lolium perenne L.): Potential effects on animal performance and challenges in determining profiles and concentrations. Animal Feed Science and Technology 277, 114960.
  • Knežević J., Stanisavljević R. (2018). Posebno ratarstvo – industrijsko i krmno bilje. Univerzitet u Prištini, Poljoprivredni fakultet, Lešak, Srbija.
  • Lee M. R. F., Colmenero J. J. O., Winters A. L., Scollan N. D., Minchin F. R (2006). Polyphenol oxidase activity in grass and its effect on plant-mediated lipolysis and proteolysis of Dactylis glomerata (cocksfoot)in a simulated rumen environment. Journal of the Science of Food and Agriculture 86, 1503-1511.
  • Loaiza P. A., Balocchi O., Bertrand A. (2017). Carbohydrate and crude protein fractions in perennial ryegrass as affected by defoliation frequency and nitrogen application rate. Grass and Forage Science 72, 556-567.
  • Makkar H. P., Becker K. (1993). Vanilin-HCl method for condensed tannins effect of organic solvents used for exctraction of tannins. Journal of Chemical Ecology 19 (84), 613-621.
  • Rigui A. P., Carvalho V., dos Santos A. L. W., Morvan Bertrand A., Prudhomme M. P., de Carvalho M. A. M., Gaspar M. (2019). Fructan and antioxidant metabolisms in plants of Lolium perenne under drought modulated by wxogenus nitric oxide. Plant Physiology and Biochemistry 145, 205-215.
  • Roupheal Y., Giordano M., Cardelli M., Cozzolino E., Mori M., Kyriacou U. C., Bonini P., Colla G. (2018). Plant and sea-weed-based extracts increase yield but differentially modulate nutritional quality of greenhouse spinach through biostimulant action. Agronomy 8, 126-141.
  • Singleton V. L., Rossi A. (1965). Colorimetry of total phenolics with phopitomolybolic-phosphotungstic acid reagent. American Journal of Enology and Vitriculture 16, 144-158.
  • Tunner L. R., Donaghy D. J., Pembleton K. G., Rawnsley R. P. (2015). Longer defoliation interval ensures expression of the “high sugar” trait in perennial ryegrass cultivars in cool temperate Tasmania, Australia. Journal of Agricultural Science 153, 995-1005.