2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023 (334-337)
АУТОР(И) / AUTHOR(S): Aleksandra Vitkovac, Tanja Pajić, Marta Bukumira, Marina Stanić, Mihailo Rabasović, Nataša Todorović
Е-АДРЕСА / E-MAIL: b1039_2022@stud.bio.bg.ac.rs
DOI: 10.46793/ICCBI23.334V
САЖЕТАК / ABSTRACT:
Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.
КЉУЧНЕ РЕЧИ / KEYWORDS:
Phycomyces, Imaging, TPEF, Machine-learning, Ilastik
ЛИТЕРАТУРА / REFERENCES:
- A.R. Fenton, T.A. Jongens, E.L.F. Holzbaur. Mitochondrial dynamics: Shaping and remodeling an organelle network. Curr Opin Cell Biol. 68 (2021) 28-36.
- T. Pajic, S. Kozakijevic, A.J. Krmpot, et al. In vivo multiphoton imaging of a filamentous fungus Phycomyces blakesleeanus: the effect of small ambient temperature increase on mitochondrial morphology and lipid droplets density. IX International School and Conference on Photonics, PHOTONICA 2023, August 28 – September 1, 2023, Belgrade-Serbia.
- M. Stanić, J. Zakrzewska, M. Hadžibrahimović, et al. Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. Res Microbiol. 164(7) (2013) 770-8.
- T. Pajić, N.V. Todorović, M. Živić, et al. Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi. Sci Rep. 12(1) (2022) 18760.