2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023 (86-90)
АУТОР(И) / AUTHOR(S): Vladimir Šaraba, Jasmina Nikodinovic-Runic, Vesna Obradović, Ivica Dimkić, Tamara Janakiev, Veselin Dragišić, Milica Ciric
Е-АДРЕСА / E-MAIL: vsaraba@imgge.bg.ac.rs
DOI: 10.46793/ICCBI23.086S
САЖЕТАК / ABSTRACT:
Targeted physiological groups of bacteria were cultivated and identified in the brackish groundwaters of Obrenovačka Banja (OB), Lomnički Kiseljak (LK) and Velika Vrbnica (VV) using biological activity reaction tests (BARTs) to assess the biocorrosion, biofouling and health risks. The highest density of iron-related, sulfate-reducing, slime-forming, facultatively anaerobic heterotrophic, denitrifying bacteria and representatives of Pseudomonas spp. was recorded in the OB sample, while the lowest density of the same physiological groups of bacteria was recorded in the LK sample. Facultatively anaerobic heterotrophic bacteria were the most abundant in the OB and LK samples, while, in contrast, heterotrophic aerobic bacteria were the most abundant in the VV sample. All tested samples were characterized by a high degree of biochemical activity associated with iron-related, sulfate-reducing, slime-forming, heterotrophic aerobic and facultatively anaerobic bacteria. Also, high biochemical activity of denitrifying bacteria was recorded in the OB sample, and the same activity of Pseudomonas species was recorded in the OB and VV samples. For OB and LK groundwaters, the highest degree of risk was estimated for biocorrosion process, while for the OB and VV occurrences, the highest degree of risk was estimated for biofouling process. The health risk was present for all examined groundwaters. Caution is warranted in further use of all investigated occurrences due to the established public health risk and an immediate revitalization of the OB, LK and VV wells is necessary.
КЉУЧНЕ РЕЧИ / KEYWORDS:
brackish groundwaters, biological activity reaction tests, Serbia.
ЛИТЕРАТУРА / REFERENCES:
- N. Mansuy., Water well rehabilitation: A practical guide to understanding well problems and solutions, USA: CRC Press, (2017).
- K.E. Murray, E.I. Manitou-Alvarez, E.C. Inniss, F.G. Healy, A.A. Bodour., Assessment of oxidative and UV-C treatments for inactivating bacterial biofilms from groundwater wells, Frontiers of Environmental Science & Engineering, 9 (2015), 39-49.
- S.A. Smith., Biofouling in Water Wells, In: J.H. Lehr, J. Keeley., (Ed.), Water Encyclopedia: Ground Water (pp. 35-38), John Wiley & Sons, Inc., (2005).
- V. Šaraba, V. Dragišić, T. Janakiev, V. Obradović, M. Ćopić, B. Knežević, I. Dimkić., Bacteriome composition analysis of selected mineral water occurrences in Serbia, Archives of Biological Sciences, 74 (2022), 67-79.
- L.C. Simões., Biofilms in drinking water, In: M. Simões, F. Mergulhão (Ed.), Biofilms in Bioengineering (pp. 157-189), USA, NY: Nova Science Publishers, Inc., (2013).
- J. Liu., Mechanism of biocorrosion caused by biofilms and its mitigation (Doctoral dissertation), USA: Ohio University, (2017).
W. Dou, D. Xu, T. Gu., Biocorrosion caused by microbial biofilms is ubiquitous around us. Microbial Biotechnology, 14 (2021), 803-805. - J. Guo, S. Yuan, W. Jiang, L. Lv, B. Liang, S.O. Pehkonen., Polymers for combating biocorrosion. Frontiers in Materials, 5 (2018), 10.
- B. Filipović., Mineralne, termalne i termomineralne vode Srbije, Belgrade: University of Belgrade, Faculty of Mining and Geology, (2003).
- D. Stojadinović, D. Isaković., Uticaj klimatskih promena na temperaturni režim mineralne vode Lomničkog kiseljaka kod Kruševca, In: S. Popović (Ed.), Water for the 21st century, Belgrade: Association for Water Technology and Sanitary Engineering (pp. 679-84), (1999).
- I. Đinđić, D. Drašković, S. Špadijer, S. Drobac, T. Vinčić, Lj. Mrkonja., Analysis of thermo-mineral water regime of „New Obrenovac Spa“ and possibility for the use of thermal water, In: A. Vranješ (Ed.), XVI Serbian Symposium on Hydrogeology (pp. 171-7), Belgrade: Universtiy of Belgrade, Faculty of Mining and Geology, (2022).
- D. Stojadinović, G. Rašula., Sadašnje stanje u pogledu mogućnosti korišćenja mineralne vode Lomničkog kiseljaka kod Kruševca, In: Z. Stevanović (Ed.), 100 years of hydrogeology in Yugoslavia, Belgrade: University of Belgrade, Faculty of Mining and Geology (pp. 197-201), (1997).
- Drycon Bioconcepts Inc (DBI), Biological Activity Reaction Tets – BARTTM, (2004). Available at: http://www.dbi.ca/BARTs/PDFs/Manual.pdf
- Official Gazzete., Rulebook on quality and other requirements for natural mineral water, natural spring water and table water 53/2005 and 43/2013, (2005/2013). Available at: http://www.pravno-informacioni-sistem.rs/SlGlasnikPortal/eli/rep/slscg/ministarstva/pravilnik/2005/53/1/reg
- World Health Organization (WHO)., Guidelines for drinking-water quality (4th edition), Geneva, Switzerland: World Health Organization, 541 p., (2011).
- National Primary Drinking Water Regulations (NPDWR) – Microorganisms, Washington, D.C: United States Environmental Protection Agency. Available at: https://www.epa.gov/ground-waterand-drinking-water/national-primary-drinking-waterregulations#Microorganisms.