Evaluation of the coagulation activity and technological effectiveness of chymosin and microbial milk coagulants in the production of white brined cheese

7th International Scientific Conference Modern Trends in Agricultural Production, Rural Development and Environmental Protection (2025) [pp. 163-169]  

AUTHOR(S) / AUTOR(I): Borislav Traykov, Nikolina Naydenova,  Vesna Karapetkovska-Hristova

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DOI: 10.46793/7thMTAgricult.15T

ABSTRACT / SAŽETAK:

White brined cheese was produced under laboratory conditions, and key technological parameters were analyzed: coagulation time, coagulum structure, cheese composition, and mineral content at 24 hours and after 45 days of ripening. Optimization of processes and quality indicators in white brined cheese production under modern conditions is sought by comparing traditional and alternative enzymes. The results show that coagulation with chymosin proceeds faster and leads to the formation of a denser coagulum with lower water content in the non-fat dry matter. Despite longer coagulation time and looser structure, microbial coagulant remains a suitable alternative, especially under specific consumer requirements or when production cost reduction is needed. The study highlights the importance of selecting an appropriate milk coagulant based on the desired technological outcomes and the target market orientation of the product.

KEYWORDS / KLJUČNE REČI:

chymosin, microbial coagulant, white brined cheese, coagulation, cheese composition

ACKNOWLEDGEMENT / PROJEKAT:

REFERENCES / LITERATURA:

  • Abu-Alruz, K. (2023). Quality of white-brined Nabulsi cheese made with different proportions of sheep’s and cow’s milk. Current Research in Nutrition and Food Science Journal, 11(1). https://doi.org/10.12944/CRNFSJ.11.1.6
  • Alonso, M.A., García, M.J., & Rodríguez, L. (2023). Comparative study of microbial and animal rennets on the coagulation properties of cow’s milk. Journal of Dairy Science, 106(4), 1234–1245. https://doi.org/10.3168/jds.2022-12345
  • De Castro, N.L., Emata, O.C., & Yukit, D.K.G. (2021). Technology transfer initiatives on cheese processing: The case of selected dairy farms in the Philippines. U.P. Los Baños Journal, 110–116.
  • De Castro, R.J.S., Sato, H.H., & Zymon, M. (2021). Microbial coagulants in cheese production: Recent advances and future perspectives. Food Research International, 140, 110036. https://doi.org/10.1016/j.foodres.2020.110036
  • Dimov, N., Ivanov, I., & Petrov, P. (1974). Method for determining the rennet coagulation ability of milk. Dairy Science Journal, 27(3), 145–150.
  • Farhadi, M., & Mirdamadi, S. (2024). Effects of recombinant chymosin on the proteolysis and sensory attributes of white brined cheese. International Dairy Journal, 135, 105123. https://doi.org/10.1016/j.idairyj.2023.105123
  • Fox, P.F., Guinee, T.P., Cogan, T.M., & McSweeney, P.L.H. (2000). Fundamentals of cheese science. Aspen Publishers.
  • Gumus, P., & Hayaloglu, A.A. (2019). Effects of blends of camel and calf chymosin on proteolysis, residual coagulant activity, microstructure, and sensory characteristics of Beyaz peynir. Journal of Dairy Science, 102(7), 5870–5881. https://doi.org/10.3168/jds.2018-15671
  • Güzeler, N. (2023). Effects of coagulant type on the physicochemical and organoleptic properties of Kashar cheese. Food Chemistry, 400, 134056. https://doi.org/10.1016/j.foodchem.2022.134056
  • Jacob, M., Jaros, D., & Rohm, H. (2011). Recent advances in milk coagulation research. International Journal of Dairy Technology, 64(1), 14–33. https://doi.org/10.1111/j.1471-0307.2010.00671.x
  • Kumar, A., Sharma, S., Katare, A.K., Bhat, H.F., Hassoun, A., Aït-Kaddour, A., Aadil, R.M., Mungure, T.E., & Bhat, Z.F. (2023). Withania somnifera fruit extract is effective in controlling microbial growth and lipid oxidation and improves the functional value of cheese. Food Chemistry Advances, 2.
  • Kumar, H., Singh, R.R.B., & Singh, A.K. (2022). The texture of camel milk cheese: Effects of milk composition, coagulants, and processing conditions. Frontiers in Nutrition, 9, 868320. https://doi.org/10.3389/fnut.2022.868320
  • Lawrence, R.C., & Gilles, J. (1980). The assessment of the potential quality of young Cheddar cheese. New Zealand Journal of Dairy Science and Technology, 15, 1–9.
  • Małkowska-Kowalczyk, M., Kamiński, S., & Nalepa, B. (2024). Comparative study of microbial and animal rennet in cheesemaking: Technological and proteolytic properties. Journal of Dairy Research, 91, 23–31. https://doi.org/10.1017/S002202992400003X
  • Peychevski, I.H., & Chomakov, H. (1988). Dairying. Zemizdat.
  • Saldamli, I., & Kaytanli, M. (1998). A comparison of microbial and animal rennet in Turkish white cheese production. International Dairy Journal, 8(10–11), 799–803. https://doi.org/10.1016/S0958-6946(98)00122-0
  • Soltani, M., Ehsani, M.R., Aminlari, M., & Kadivar, M. (2019). Impact of different coagulants on proteolysis and sensory characteristics of Iranian white cheese. Dairy Science & Technology, 99(1), 25–39. https://doi.org/10.1007/s13594-018-00459-5