2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023 (511-514)
АУТОР(И) / AUTHOR(S): Jovica Tomović, Perica Vasiljević, Aleksandar Kočović, Nedeljko Manojlović
Е-АДРЕСА / E-MAIL: jovicatomovic2011@gmail.com
DOI: 10.46793/ICCBI23.511T
САЖЕТАК / ABSTRACT:
The lichens synthesize a large number of secondary metabolites and most of these compounds are unique to lichen. The present study provides data concerning the chemical characterization and determination of total phenolic and flavonoid contents of lichen extracts of Umbilicaria crustulosa. Chemical profiling of the extracts was done by high-performance liquid chromatography coupled with a UV detector (HPLC/UV), while the determination of total phenolic and flavonoid contents was done by the spectrophotometric method. HPLC analysis of the acetone and methanol extracts of U. crustulosa lichen revealed the presence of the methyl-orselinate, lecanoric acid, crustinic acid, haematommic acid, gyrophoric acid, methyl lecanorate, physodic acid, atranorin and chloroatranorin as the main compounds. The most abundant compounds of the acetone and methanol extracts were the tri-depside gyrophoric acid (59.27 % and 58.32 %) and didepside lecanoric acid (7.41 % and 11.43 %). The results of the total phenolic content (TPC) and total flavonoid content (TFC) show that the acetone extract had higher values of TPC (205.46 mg GA/g) and TFC (290.18 mg RE/g; 160.50 mg QE/g). The investigated extracts of the lichen U. crustulosa can be used as a significant source of biologically active compounds.
КЉУЧНЕ РЕЧИ / KEYWORDS:
Lichens, Phenols, HPLC, Umbilicaria
ЛИТЕРАТУРА / REFERENCES:
- M.P. Nelsen, A. Gargas., Phylogenetic distribution and evolution of secondary metabolites in the lichenized fungal genus Lepraria (Lecanorales: Stereocaulaceae), Nova Hedwigia, 86 (2008) 115–131.
- B. Ranković., Lichen Secondary Metabolites: Bioactive properties and pharmaceutical potential, Springer, 1 (2014) 1-26.
- M. Kosanić, B. Ranković, T. Stanojković., Antioxidant, antimicrobial, and anticancer activity of 3 Umbilicaria species, Journal of food science, 77 (2012) 20-25.
- V.L. Singleton, R. Orthofer, R.M. Lamuela-Raventós., Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299 (1999) 152-178.
- S. Aryal, M.K. Baniya, K. Danekhu, P. Kunwar, R. Gurung, N. Koirala., Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal, Plants, 8 (2019) 96.
- B. Posner, G.B. Feige, S. Huneck., Studies on the chemistry of the lichen genus Umbilicaria Hoffm, Zeitschrift für Naturforschung C, 47 (1992) 1-9.
- J.A. Elix, K.L. Gaul., The interconversion of the lichen depsides para-and meta-scrobiculin, and the biosynthetic implications, Australian Journal of Chemistry, 39 (1986) 613-624.
- B. Ranković, M. Kosanić, N. Manojlović, A. Rančić, T. Stanojković., Chemical composition of Hypogymnia physodes lichen and biological activities of some its major metabolites. Medicinal Chemistry Research, 23 (2014) 408-416.