AUTHOR(S) / AUTOR(I): Tatjana Đorđević 
, Mirjana Antov
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DOI: https://doi.org/10.46793/SBT26.617D
ABSTRACT / SAŽETAK:
Wheat chaff was pretreated by ultrasonic, hydrothermal, and alkali-assisted hydrothermal pretreatments to enhance the enzymatic hydrolysis of arabinoxylan component for xylooligosaccharide production. FTIR analyses showed structural changes in wheat chaff after pretreatments, including delignification, removal of acetyl and phenolic esters, and disruption of lignin–hemicellulose linkages. Hydrolysis of arabinoxylan in pretreated wheat chaff with endo-xylanase produced approx. 120, 290, and 400 mg/g of xylooligosaccharides after ultrasonic, hydrothermal, and alkali-assisted hydrothermal pretreatments, respectively, compared to 100 mg/g from untreated chaff.
KEYWORDS / KLJUČNE REČI:
xylooligosaccharides, endo-xylanase, hydrolysis, wheat chaff, pretreatment
ACKNOWLEDGEMENT / PROJEKAT:
The research presented in this article is part of Program (Number 451-03-137/2025-03/ 200134) financially supported by the Ministry of Education, Science and Technological Development, Republic of Serbia.
REFERENCES / LITERATURA:
- Aachary, A. A., & Prapulla, S. G. (2009). Value addition to corncob: production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154. Bioresource Technology, 100: 991-995.
- Azizan A., Jusri N. A. A., Azmi I. S., Abd Rahman M. F., Ibrahim N., Jalil R. (2022). Emerging lignocellulosic ionic liquid biomass pretreatment criteria/strategy of optimization and recycling—short review with infrared spectroscopy analytical know-how. Materials Today: Proceedings. 63: S359–S367.
- Bussemaker, M. J., & Zhang, D. (2013). Effect of ultrasound on lignocellulosic biomass as a pretreatment for biorefinery and biofuel applications. Industrial and Engineering Chemistry Research, 52: 3563–3580.
- De Mello Capetti, C. C., Pellegrini, V. O. A., Santo, M. C. E., Cortez, A. A., Falvo, M., da Silva Curvelo, A. A., … & Polikarpov, I. (2023). Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies. Carbohydrate Polymers, 299: 120174.
- Hýsek Š., Podlena M., Bartsch H., Wenderdel C., Böhm M. (2018). Effect of wheat husk surface pre-treatment on the properties of husk-based composite materials. Industrial Crops and Products. 125: 105–113.
- Korai R. M., Li X. (2020). Effect of ultrasonic assisted KOH pretreatment on physiochemical characteristic and anaerobic digestion performance of wheat straw. Chinese Journal of Chemical Engineering. 28(9): 2409–2416.
- Rao S., Madhushree M., Bhat K. S. (2024). Characteristics of surface modified sugarcane bagasse cellulose: application of esterification and oxidation reactions. Scientific Reports. 14(1): 24136.
- Saratale G. D., Saratale R. G., Varjani S., Cho S. K., Ghodake G. S., Kadam A., Mulla S. I., Bharagava R. N., Kim D. S., Shin H. S. (2020). Development of ultrasonic aided chemical pretreatment methods to enrich saccharification of wheat waste biomass for polyhydroxybutyrate production and its characterization. Industrial Crops and Products. 150: 112425.
- Sharma D., Sharma V., Tsai M. L., Yadav A., Nargotra P., Sun P. P., Dong C. D. (2025). Improved xylooligosaccharides production from xylan extracted using ultrasonic-assisted alcoholic deep eutectic solvent pretreatment of pineapple leaf waste. Industrial Crops and Products. 224: 120250.
- Sharma S., Nargotra P., Sharma V., Bangotra R., Kaur M., Kapoor N., Bajaj B. K. (2021). Nanobiocatalysts for efficacious bioconversion of ionic liquid pretreated sugarcane tops biomass to biofuel. Bioresource Technology. 333: 125191.