DIJAGNOZA KETOZE KOD MLEČNIH KRAVA

Savetovanje o biotehnologiji sa međunarodnim učešćem (Čačak: 27; 2022) (str. 219-228)

АУТОР(И): Radojica Đoković, Marko Cincović, Milun Petrović, Miloš Petrović, Boban Jašović, Biljana Anđelić, Miroslav Lalović

Е-АДРЕСА: radojicadjokovic@gmail.com

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DOI: 10.46793/SBT27.219DJ

САЖЕТАК:

Cilj ovog rada je da opiše načine dijagnostikovanja ketoze, uključujući laboratorijske i brze (cowside) testove. Standardni “zlatni test” za dijagnozu subkliničke ketoze (SKK) kod mlečnih krava jeste laboratorijsko merenje koncentracije beta-hidoksibuterne kiseline (BHB) u krvnom serumu ili krvnoj plazmi. Najčešće korišćene granične vrednosti za dijagnozu subkliničke ketoze jesu vrednosti za BHB u krvi veće od 1,2 mmol/L ili veće od 1,4 mmol/L, dok za kliničku ketozu one iznose oko 3,0 mmol/L. Cowside test za određivanje koncentracije BHB u krvi jeste jednostavni ručni uređaj (ketometar) i može se upotrebljavati na farmi, a ovaj test ima visoki procenat (˃95%) oestljivosti i specifičnosti u odnosu na druge cowside testove i može zameniti laboratorijska ispitivanja u dijagnozi ketoze. Cowside BHB test, odnosno ketolac BHB test trakice sa graničnim vrednostima većim od 200 μmol/L za BHB u mleku je potencijalno korisno sredstvo za rutinski pregled stada mlečnih krava na početku laktacije za dijagnozu ketoze.

КЉУЧНЕ РЕЧИ:

mlečne krave, ketoza, laboratorijski testovi, cowside testovi

ЛИТЕРАТУРА:

  • Brunner N., Groeger S., Raposo J.C., Bruckmaier R.M., Gross J.J. (2019): Prevalence of subclinical ketosis and production diseases in dairy cows in Central and
  • South America, Africa, Asia, Australia, New Zealand, and Eastern Europe. Translational Animal Science Vol. 3, pp. 19–27.
  • Carrier J., Steward S., Godden S., Fetrow J., Rapnicki P. (2004): Evaluation and use of three cowside tests for detection of subclinical ketosis in early postpartum cows. Journal of Dairy Science, Vol.87, pp. 3725-3735.
  • Cook N.B., Waed W.R.., Dobson H. (2001): Concentrations of ketones in milk in early lactation, and reproductive performance of dairy cows. Veterinary Record, vol. 148, pp. 769-772.
  • De Roos A.P.W., Van Den Bijgaarth J., Hrrlyk J., De Jong G. (2007): Screening for subclinical ketosis in dairy cattle by Fourier transform infrared spectrometry. Journal of Dairy Science, Vol.90, pp. 1761-1766.
  • Dirksen G., Breitner W. (1993): A new quick test for semiquantitative determination of betahydroxybutyric acid in bovine milk. Journal of Veterinary Medicine A, Vol.40, pp. 779-784.
  • Djokovic R., Kurcubic V, Ilic Z, Cincovic M., Fratric N., Stanimirovic Z., Petrovic M.D., Petrovic M.P. (2013): Evaluation of the metabolic status of Simmental dairy cows in early and mid lactation. Animal Science Papers and Reports, 31: 101- 110.
  • Djokovic R., Jozek J., Cincovic M., Nemecc M., Belic B., Klinkom M., Staric J. (2018): Beta-hydroxybutyrate in milk as screening test for subclinical ketosis in dairy cows. pp. 127. The 30th World Buiatrics Congress, August 28 to September 1, Sapporo, Japan.
  • Djokovic R., Ilic Z., Kurcubic V., Petrovic M., Cincovic M., Petrovic P.M., Caro- Petrovic V. (2019): Diagnosis of subclinical ketosis in dairy cows. Biotechnology in Animal Husbandry, Vol. 35, No.2, pp. 111-125. ISSN 1450- 9156, UDC 636.09’65 https://doi.org/10.2298/BAH1901001P.
  • Duffield T. (2000): Subclinical ketosis in lactating dairy cattle. Veterinary Clinics of North America: Food Animal Practice, Vol.16, pp.231-253.
  • Duffield T., Bagg R. (2002): Herd level indicators for the prediction of high-risk dairy herds for subclinical ketosis. Pp. 175-176 in Proc. Proceeding of American Association of Bovine Practitioner, Rome, GA.
  • Eicher R. (2004): Evaluation of the metabolic and nutritional situation in dairy herds: Diagnostic use of milk components. 23rd World Buiatrics Congress, Québec, Canada.
  • Enjalbert F., Nicot M. C., Bayourthe A. C., Monculon R. (2001): Ketone bodies in  milk and blood of dairy cows: Relationship between concentrations and utilization for detection of subclinical ketosis. Journal of Dairy Science, Vol. 84, pp. 583-589.
  • Geishauser T., Leslie K., Kelton K., Duffield T. (1998): Evaluation of five cowside tests for use with milk to detect subclinical ketosis in dairy cows. Journal of Dairy Science, Vol.81, pp. 438-443.
  • Geishauser T., Leslie K., Ten Hag, J., Bashiri A. (2000): Evaluation of eight cow-side ketone tests in milk for detection of subclinical ketosis in dairy cows. Journal of Dairy Science, Vol.83, pp.296-299.
  • Geishauser T., Leslie K., Kelton K., Duffield T. (2001): Monitoring for subclinical ketosis in dairy herds. Compendium, S65-S71.
  • Hansen P.W. (1999): Screening of dairy cows for ketosis by use of infrared spectroscopy and multivariate calibration. Journal of Dairy Science, Vol.82, pp. 2005-2010.
  • Heurer C., Luinge H.J., Lutz E.T.G., Schukken Y.H., Van Der Maas J.H., Wilmink H. Noordhuizen T.M. (2001): Determination of acetone in cow milk by fourier transform infrared spectroscopy for the detection of subclinical ketosis. Journal of Dairy Science, Vol. 84, pp. 575-582.
  • Herdt T.H. (2000): Ruminant adaptation to negative energy balance: Influences on the etiology of ketosis and fatty liver. Journal of Dairy Science, Vol. 16, pp. 215- 230.
  • Holtenius P., Holtenius K. (1996): New aspects of ketone bodies in energy metabolism of dairy cows: A review. Journal of Veterinary Medicine A, Vol. 43, pp. 579-587.
  • Heuwieser W., Falkenberg U., Iwersen M., Voigtsberger R., Padberg W., (2007): Evaluation and use of an automated human β-hydroxybuturate (BHBA) test for cowside detection of subclinical ketosis in dairy cattle. Proceedings of the Fortieth Annual Conference, American Association of Bovine Practitioners, Vancouver, British Columbia, Canada, pp: 253-254.
  • Iwersen M., Falkenber U., Voigtsberger R., Forderung D., Heuwieser W. (2009): Evaluation of an electronic cowside test to detect subclinical ketosis in dairy cows. Journal of Dairy Science, Vol. 92, pp. 2618-2624.
  • Jozek J., Cincovic M., Nemec M., Belic B., Djokovic R., Klinkom M., Staric J. (2017): Beta-hydroxybutyrate in milk as screening test for subclinical ketosis in dairy cows. Polish Journal of Veterinary Science, Vol. 20, pp. 507-512.
  • Jenkins NT, Peña G, Risco C, Barbosa CC, Vieira-Neto A, Galvão KN. (2015): Utility of inline milk fat and protein ratio to diagnose subclinical ketosis and to assign propylene glycol treatment in lactating dairy cows. The Canadian veterinary journal 2015 Aug; 56(8):850-4. PMID: 26246632; PMCID: PMC4502854.
  • Kinoshita A, Wolf C., Zeyner A. (2010): Studies on the incidence of hyperketonemia with and without hyperbilirubinaemia in cows in Mecklenburg-Vorpommern (in Germany) in the course of the year. Tieraerztliche Praxis, Vol.38, pp. 7-15.
  • Konkol K., Godden S., Rapnicki P., Overton M., Rollin E. (2009): Validation of a rapid cowside test for the measurement of blood betahydroxybutyrate in fresh cows. Minnesota Dairy Health Conference, pp: 86-87.
  • Larsen T. Nielsen N. (2005): Fluorometric determination of β- hydroxybutyrate in milk and blood plasma. Journal of Dairy Science, Vol.88, pp. 2004-2009.
  • Nielen M., Aart M.G.A., Jonker A.G.M., Wesing T., Schukken Y.H. (1994): Evaluation of two cowside tests for the detection of subclinical ketosis in dairy cows. Canadian Veterinary Journal, Vol. 35, pp. 229-232.
  • Oetzel G.R. (2004): Monitoring and testing dairy herds for metabolic disease. Veterinary Clinics of North America: Food Animal Practice, Vol.20, pp. 651- 674.
  • Oetzel G.R. (2007): Herd level ketosis- Diagnosis and risk factors. Preconference Seminar 7C: Dairy Herd Problem Investigation Strategies: Transition Cow Troubleshooting, American association of bovine practitioners 40th Annual Conference, pp. 67-91, September 19, Vancouver, BC, Canada
  • Ospina P.A., Nydam D.V., Stoko T., Overton T.R., (2010): Evaluation of nonesterified fatty acids and β-hydroxybutyrate in transition dairy cattle in the northeastern United States: Critical thresholds for prediction of clinical diseases. Journal of Dairy Science, Vol.93, pp. 546-554.
  • Osborrne T.M., Leslie K.E., Duffield T., Petersson C.S., Ten Hag J., Okada Y. (2002): Evaluation of Keto-Test in urine and milk for the detection of subclinical ketosis in periparturient Holstein dairy cattle. pp. 188-189 in  Proc. Proceedings of the 35th Conference of the American Association of Bovine Practitioners, Rome, GA.
  • Seifi H.A., Le Blanc S.J., Leslie K.E. Duffield T.F. (2011): Metabolic predictors of post- partum disease and culling risk in dairy cattle. Veterinary Journal, Vol.188, pp. 216-220.
  • Tyopponen J., Kauppinen K. (1980): The stability and automatic determination of ketone bodies in blood samples taken in field conditions. Acta Veterinaria Scandinavica, Vol.21, pp. 55-61.
  • Williamson D.H., Meellanby J. (1974): D-(-)3-hydroxybutyrate. In methods of enzymatic analysis, 1st ed., Academic Press, New York, pp 1836-1837.
  • Whitaker D.A. (1997): Interpretation of metabolic profiles in dairy cows. Cattle Practitioner, Vol. 5, pp. 57- 60.
  • Van Knegsel A.T.M., Van Der Drift S.G.A., Horneman M., De Roos A.P.W., Kemp B. Graat E.A.M. (2010): Ketone body concentration in milk determined by fourier transform infrared spectroscopy: Value for the detection of hyperketonemia in dairy cows. Journal Dairy Science, Vol. 93, pp. 3065-3069.
  • Venkatewarlu K., Choudhuri P.C. (2001): Detection of acetone in milk: Qualitative and quantitative salicylaldehyde test for diagnosis of subclinical ketosis in dairy cows. Indian Veterinary Journal, Vol.78, pp. 335-336.
  • Voyvoda H., Erdogan H. (2010): Use of a hand-held meter for detecting subclinical ketosis in dairy cows. Research Veterinary Science, Vol. 89, pp. 344-351.
  • Zhang Z., Liu G., Wang H., Li. X., Wang Z. (2012): Detection of subclinical ketosis in dairy cows. Pakistan Veterinary Journal, Vol. 32, pp. 156-160.