Biological activity and traditional use of parasiticplants of the genus „Cuscuta“ in Bulgaria

Етноботаника 5 (2025), Том 2 (стр. 209-235)

АУТОР(И) / AUTHOR(S): Lyuben Zagorchev, Denitsa Teofanova

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DOI: 10.46793/EtnBot25.209Z

САЖЕТАК / ABSTRACT:

Cuscuta spp., or dodders, are widely distributed obligate parasitic plants with a strong economic impact due to a significant reduction in crop plants’ yield. However, they are also known as prominent medicinal plants in ethnobotanical studies, especially in Asian countries. The phytochemical composition of Cuscuta extracts, rich in alkaloids, flavonoids and various other bioactive compounds, determines a broad spectrum of pharmacological effects, including hepatoprotective, anti-inflammatory and wound-healing effects, etc., while a strong antioxidant effect has also been noted.  The genus is represented in Bulgaria by up to ten species, some of which are included as medicinal plants in the national legislation. There are also several reports on the traditional use in ethnomedicinal practices in the country. However, particular investigations on the phytochemistry and pharmacological effects are especially scarce. Recent studies suggested that representatives of the genus in Bulgaria might represent a rich and diverse source of bioactive compounds, but more data are needed to characterise this diversity.

КЉУЧНЕ РЕЧИ / KEYWORDS:

antioxidant activity, dodders, parasitic plants, flavonoids

ACKNOWLEDGEMENT / ПРОЈЕКАТ:

This study is financed by the European Union-NextGenerationEU, through the National Recovery and Resilience Plan of the Republic of Bulgaria, project No BGRRP-2.004-0008.

REFERENCES / ЛИТЕРАТУРА:

• Ahmad, A., Tandon, S., Xuan, T. D., & Nooreen, Z. (2017). A Review on Phytoconstituents and Biological activities of Cuscuta species. Biomedicine & Pharmacotherapy, 92, 772-795.
  https://doi:10.1016/j.biopha.2017.05.124
• Assyov, B., & Petrova, A. (2012). Conspectus of the vascular plants in Bulgaria, Sofia, Bulgarian Biodiversity Foundation.
• Atala, E., Fuentes, J., Wehrhahn, M. J., & Speisky, H. (2017). Quercetin and related flavonoids conserve their antioxidant properties despite undergoing chemical or enzymatic oxidation. Food Chemistry, 234, 479-485. https://doi:10.1016/j.foodchem.2017.05.023
• Baráth, K., Lengyel, A., & Csiky, J. (2025). Host specificity of Cuscuta species: is it a cause or a consequence of the habitat preference? Plant and Soil, 1-13. https://doi.org/10.1007/s11104-02507210-2
• Benvenuti, S., Dinelli, G., Bonetti, A., & Catizone, P. (2005). Germination ecology, emergence and host detection in Cuscuta campestris. Weed Research, 45(4), 270-278. https://doi.org/10.1111/j.1365-3180.2005.00460.x
• Bhavya, B.C., Haridas, M. (2017). Anti-inflammatory Molecules: Immune System Mediators. In:
  S. Sugathan, N. Pradeep, S.Abdulhameed (Eds), Bioresources and Bioprocess in Biotechnology (pp. 235-268). Springer, Singapore. https://doi.org/10.1007/978-981-10-4284-3_10
• Bonvicini, F., Antognoni, F., Mandrone, M., Protti, M., Mercolini, L., Lianza, M., Gentilomi, G., & Poli, F. (2017). Phytochemical analysis and antibacterial activity towards methicillin-resistant Staphylococcus aureus of leaf extracts from Argania spinosa (L.) Skeels. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 151(4), 649-656.
  https://doi.org/10.1080/11263504.2016.1190418
• Chakarova, B., Zagorchev, L., Pachedjieva, K., Tosheva, A., Zagorcheva, T., Rusanov, K., & Teofanova, D. (2025). Analysis of Variations in the Flavonoid Profiles of Cuscuta campestris and Cuscuta epithymum in Bulgaria as a Potential Chemotaxonomical Marker. Plants, 14(8), 1220. https://doi.org/10.3390/plants14081220
• Costea, M., García, M. A., & Stefanović, S. (2015). A phylogenetically based infrageneric classification of the parasitic plant genus Cuscuta (dodders, Convolvulaceae). Systematic Botany, 40(1), 269-285. https://doi.org/10.1600/036364415X686567
• Dell’Albani, P., Di Marco, B., Grasso, S., Rocco, C., & Foti, M. C. (2017). Quercetin derivatives as potent inducers of selective cytotoxicity in glioma cells. European Journal of Pharmaceutical Sciences, 101, 56-65. https://doi.org/10.1016/j.ejps.2017.01.036
• Dikova, B. (2006). Establishment of tobacco rattle virus (TRV) in weeds and Cuscuta. Biotechnology & Biotechnological Equipment, 20(3), 42-48. https://doi.org/10.1080/13102818.2006.10817379
• Dimitrov, D., Kurteva, M., & Zahriev, D. (2012). Flora and vegetation of the Dervisha Managed Reserve, Bulgaria. Phytologia Balcanica, 18, 49-57.
• Dimitrova, T. (2004). Check of Amaranthus blitoides W. var. Reverchoni Th.-an element of the control of Cuscuta epithymum Murr in lucerne (Medicago sativa L.). Bulgarian Journal of Agriacultural Science, 10, 579-582
• Dimitrova, T. (2011). Study about the Control of Cuscuta spp. in Alfalfa Medicago sativa L. Plant Science (Bulgaria) 48, 569-575.
• Donnapee, S., Li, J., Yang, X., Ge, A.-h., Donkor, P. O., Gao, X.-m., & Chang, Y.-x. (2014). Cuscuta chinensis Lam.: A systematic review on ethnopharmacology, phytochemistry and pharmacology of an important traditional herbal medicine. Journal of ethnopharmacology, 157, 292-308. http://dx.doi.org/10.1016/j.jep.2014.09.032
• Gangarde, P., Bhatt, S., & Pujari, R. (2025). Assessment of Neuroprotective Potential of Cuscuta reflexa in Aluminium Chloride-Induced Experimental Model of Alzheimer’s Disease: In Vitro and In Vivo Studies. Journal of Trace Elements in Medicine and Biology, 127612.
  https://doi.org/10.1016/j.jtemb.2025.127612
• Haidar, M., Orr, G., & Westra, P. (1998). The response of dodder (Cuscuta spp.) seedlings to phytohormones under various light regimes. Annals of Applied Biology, 132(2), 331-338.
  https://doi.org/10.1111/j.1744-7348.1998.tb05208.x
• Hajimehdipoor, H., Amin, G. R., Adib, N., Rastegar, H., & Shekarchi, M. (2012). Development of a validated HPLC method for the simultaneous determination of flavonoids in Cuscuta chinensis Lam. by ultra-violet detection. DARU Journal of Pharmaceutical Sciences, 20(1), 57.
  https://doi.org/10.1186/2008-2231-20-57
• Heide-Jørgensen, H. (2008). Parasitic flowering plants, Leiden – Boston, Brill.
• Hibberd, J., Bungard, R., Press, M., Jeschke, W., Scholes, J., & Quick, W.(1998). Localization of photosynthetic metabolism in the parasitic angiosperm Cuscuta reflexa. Planta, 205, 506-513.
• Jiang, Y., Lu, Y., Zhang, Y.-Y., & Chen, D.-F. (2014). Anti-complementary constituents of Houttuynia cordata and their targets in complement activation cascade. Natural Product
  Research, 28(6), 407-410. https://doi.org/10.1080/14786419.2013.869693
• Li, Y., Yao, J., Han, C., Yang, J., Chaudhry, M. T., Wang, S., Liu, H., & Yin, Y. (2016). Quercetin, inflammation and immunity. Nutrients, 8(3), 167. https://doi.org/10.3390/nu8030167
• Nedelcheva, A., & Draganov, S. (2014). Bulgarian medical ethnobotany: the power of plants in pragmatic and poetic frames. In: A. Pieroni, C. Quave (Eds), Ethnobotany and Biocultural
  Diversities in the Balkans (p.p. 45-65). Springer, New York, NY. https://doi.org/10.1007/978-14939-1492-0_4
• Pavlova, D., Kozuharova, E., & Dimitrov, D. (2003). A floristic catalogue of serpentine areas in the eastern Rhodope mountains (Bulgaria). Polish Boanical Journal, 48, 21-41.
• Pourcel, L., Routaboul, J.-M., Cheynier, V., Lepiniec, L., & Debeaujon, I. (2007). Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends in plant science, 12(1), 29-36. https://doi.org/10.1016/j.tplants.2006.11.006
• Runyon, J. B., Mescher, M. C., & De Moraes, C. M. (2006). Volatile chemical cues guide host location and host selection by parasitic plants. Science, 313(5795), 1964-1967.
  https://doi.org/10.1126/science.1129139
• Seo, D. J., Jeon, S. B., Oh, H., Lee, B.-H., Lee, S.-Y., Oh, S. H., Jung, J. Y., & Choi, C. (2016).
  Comparison of the antiviral activity of flavonoids against murine norovirus and feline calicivirus.
  Food Control, 60, 25-30. https://dx.doi.org/10.1016/j.foodcont.2015.07.023
• Shu, J., Li, L., Yu, H., & Zhang, D. (2021). Fertility-enhancing potential of ethanol extract of
  Cuscuta chinensis seeds in a rat model of unilateral cryptorchidism. Tropical Journal of
  Pharmaceutical Research, 20(5), 995-1002. https://doi.org/10.4314/tjpr.v20i5.16
• Singh, A., Singh, V., Ananthan, R., & Kumar, B. D. (2022). Evaluation of immunomodulatory and antioxidant properties of traditional Kwath, conventional extracts of plants Cocculus hirsutus and Cuscuta reflexa–in vitro & ex vivo studies. Journal of Ayurveda and Integrative Medicine, 13(1), 100537. https://doi.org/10.1016/j.jaim.2021.100537
• Stanilova, S. A., Zhelev, Z. D., & Dobreva, Z. G. (2000). Preliminary studies on the immunomodulatory effect of the C3 binding glycoprotein isolated from Cuscuta europea. International journal of immunopharmacology, 22(1), 15-24. https://doi.org/10.1016/S01920561(99)00060-0
• Stanilova, S. A., Dobreva, Z. G., Slavov, E. S., & Miteva, L. D. (2005). C3 binding glycoprotein from Cuscuta europea induced different cytokine profiles from human PBMC compared to other plant and bacterial immunomodulators. International immunopharmacology, 5(4), 723-734.
  https://doi.org/10.1016/j.intimp.2004.12.003
• Stoyanov, K., Raycheva, T., & Cheschmedzhiev, I. (2021). Key to the native and foreign vascular plants in Bulgaria. Plovdiv, Agricultural University Plovdiv Academic Press.
• Stoyanov, S. (2005). The vascular flora of the catchment basin of the river Roussenski Lom (Bulgaria) in the beginning of the 21st century. Flora Mediterranea, 15, 351-383.
• Suresh, V., Sruthi, V., Padmaja, B., & Asha, V. (2011). In vitro anti-inflammatory and anticancer activities of Cuscuta reflexa Roxb. Journal of ethnopharmacology, 134(3), 872-877.
  https://doi.org/10.1016/j.jep.2011.01.043
• Teofanova, D., Lozanova, Y., Lambovska, K., Pachedjieva, K., Tosheva, A., Odjakova, M., & Zagorchev, L. (2022). Cuscuta spp. populations as potential reservoirs and vectors of four plant viruses. Phytoparasitica, 50(3), 555-566. https://doi.org/10.1007/s12600-022-00981-9
• Ye, M., Li, Y., Yan, Y., Liu, H., & Ji, X. (2002). Determination of flavonoids in Semen Cuscutae by RP-HPLC. Journal of pharmaceutical and biomedical analysis, 28(3), 621-628.
  https://doi.org/10.1016/S0731-7085(01)00672-0
• Yen, F.-L., Wu, T.-H., Lin, L.-T., & Lin, C.-C. (2007). Hepatoprotective and antioxidant effects of Cuscuta chinensis against acetaminophen-induced hepatotoxicity in rats. Journal of ethnopharmacology, 111(1), 123-128. https://doi.org/10.1016/j.jep.2006.11.003
• Yen, F.-L., Wu, T.-H., Lin, L.-T., Cham, T.-M., & Lin, C.-C. (2008). Concordance between antioxidant activities and flavonol contents in different extracts and fractions of Cuscuta chinensis. Food Chemistry, 108(2), 455-462. https://doi.org/10.1016/j.foodchem.2007.10.077
• Yoshida, S., Cui, S., Ichihashi, Y., & Shirasu, K. (2016). The haustorium, a specialized invasive organ in parasitic plants. Annual review of plant biology, 67(1), 643-667. https://doi.org/10.1146/annurev-arplant-043015-111702
• Zare, G., & Dönmez, A. (2020). Cuscuta campestris Yunck. morphology, anatomy and traditional use in Turkey. Hacettepe University Journal of the Faculty of Pharmacy, 40(1), 1-10.
• Zhekova, E., Petkova, D., & Ivanova, I. (2014). Smicronyx smreczynskii F. Solari, 1952 (Insecta: Curculionidae): Possibilities for Biological Control of Two Cuscuta species (Cuscutaceae) in District of Ruse. Acta Zoologica Bulgarica, 66(3), 431-432.