1st International Conference on Chemo and BioInformatics, ICCBIKG 2021, (383-386)
AUTHOR(S) / АУТОР(И): S. Jovanović-Stević, J. Bogojeski, A. Caković, B. Petrović
E-ADRESS / Е-АДРЕСА: snezanaj@kg.ac.rs, jovana.bogojeski@pmf.kg.ac.rs, angelina.petrovic@pmf.kg.ac.rs, biljana.petrovic@pmf.kg.ac.rs
DOI: 10.46793/ICCBI21.383JS
ABSTRACT / САЖЕТАК:
The application of transition metal complexes as chemotherapeutics has been presented throughout the history. Platinum-based drugs are widely used as anticancer agents with a broad range of antitumor activities. The study of the substitution reactions of metal complexes with nitrogen containing biomolecules can help to develop new antitumor drugs with improved characteristics. Kinetics of the substitution reactions of [Pt(caffeine)2Cl2] and [Pd(caffeine)2Cl2] (caffeine = 1,3,7-trimethylxanthine) complexes with biologically important ligands such as 9-methylguanine (9-MetGua) and guanosine-5’-monophosphate (5’-GMP) were studied by UV-Vis spectrophotometry and by stopped-flow technique. Kinetics measurements were performed under the pseudo-first order conditions at 37 °C and pH = 7.2 (25 mM Hepes buffer) in addition of 50 mM NaCl. The obtained results showed that all substitution reactions undergo the two reaction steps giving the [M(caffeine)2(Nu)2] (M = Pd(II) or Pt(II) and Nu = 5′-GMP or 9-MetGua) as the reaction product. Additionally, [Pd(caffeine)2Cl2] complex was more reactive compared to analogue platinum-complex, while 9-MetGua reacted faster than 5’-GMP.
KEY WORDS / КЉУЧНЕ РЕЧИ:
Pd(II), Pt(II), Caffeine, Kinetics
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