DNA/BSA BINDING STUDY OF DINUCLEAR GOLD(III) COMPLEXES WITH AROMATIC NITROGEN-CONTAINING HETEROCYCLES AS BRIDGING LIGANDS

1st International Conference on Chemo and BioInformatics, ICCBIKG  2021, (312-315)

AUTHOR(S) / АУТОР(И): Tina P. Andrejević, Darko P. Ašanin, Nada D. Savić, Nevena Lj. Stevanović, Miloš I. Djuran, Biljana Đ. Glišić

E-ADRESS / Е-АДРЕСА: tina.andrejevic@pmf.kg.ac.rs, nevena.stevanovic@pmf.kg.ac.rs, biljana.glisic@pmf.kg.ac.rs, darko.asanin@uni.kg.ac.rs, nada.savic@kg.ac.rs, milos.djuran@pmf.kg.ac.rs

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DOI: 10.46793/ICCBI21.312A

ABSTRACT / САЖЕТАК:

In recent decades, a special attention has been devoted to gold(III) complexes as potential antitumor agents due to their structural similarity to platinum(II) complexes. One of the possible mechanisms of the mode of antitumor activity of gold(III) complexes could include their interaction with DNA. However, the effectiveness of the therapeutic agents also depends on the degree of its binding to proteins present in the blood plasma, because, in this way, it is transported to the cell. Considering this, we investigated the interactions of three dinuclear gold(III) complexes of the general formula [{AuCl3}2(μ– L)], L = 4,4’-bipy (4,4’-bipyridine, Au1), bpe (1,2-bis(4-pyridyl)ethane, Au2) and dpe (1,2-bis(4- pyridyl)ethene, Au3) with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA). The main aim of the study was to evaluate the binding affinities of gold(III) complexes Au1–3 towards these biomolecules for possible insights on their mode of biological activity. The values of binding constants (KA) of Au1–3 to ct-DNA are higher than those for BSA, indicating greater affinity of the complexes towards this nucleic acid. The partition coefficient (logP) value for Au1 is higher compared to the corresponding values for the other two complexes, what is in accordance with a higher cellular uptake efficiency of this complex.

KEY WORDS / КЉУЧНЕ РЕЧИ:

gold(III) complexes, aromatic N-heterocycles, DNA/BSA interactions, lipophilicity

  • REFERENCES / ЛИТЕРАТУРА:
  • T.P. Andrejević, B.Đ. Glišić, M.I. Djuran., Amino Acids and Peptides as Versatile Ligands in the Synthesis of Antiproliferative Gold Complexes, Chemistry, 2 (2020) 203-218.
  • S.J. Berners-Price, A. Filipovska., Gold compounds as therapeutic agents for human diseases, Metallomics, 3 (2011) 863-873.
  • B.Đ. Glišić, N.D. Savić, B. Warżajtis, L. Djokic, T. Ilic-Tomic, M. Antić, S. Radenković, J. Nikodinovic-Runic, U. Rychlewska, M.I. Djuran., Synthesis, structural characterization and biological evaluation of dinuclear gold(III) complexes with aromatic nitrogen-containing ligands: antimicrobial activity in relation to the complex nuclearity, MedChemComm, 7 (2016) 1356-1366.
  • C.A. Puckett, J.K. Barton., Methods to explore cellular uptake of ruthenium complexes, Journal of the American Chemical Society, 129 (2007) 46-47.
  • Y.-Q. Wang, H.-M. Zhang, G.-C. Zhang, W.-H. Tao, S.-H. Tang., Interaction of the flavonoid hesperidin with bovine serum albumin: A fluorescence quenching study, Journal of Luminescence, 126 (2007) 211-218.
  • Shi, C. Guo, Y. Sun, Z. Liu, F. Xu, Y. Zhang, Z. Wen, Z. Li., Interaction between DNA and microcystin-LR studied by spectra analysis and atomic force microscopy, Biomacromolecules, 12 (2011) 797-803.