1st International Conference on Chemo and BioInformatics, ICCBIKG 2021, (304-307)
AUTHOR(S) / АУТОР(И): Milan V. Žižić, Miroslav Z. Živić, Kristina D. Atlagić, Maja A. Karaman, Joanna Zakrzewska
E-ADRESS / Е-АДРЕСА: email@example.com, firstname.lastname@example.org, email@example.com, firstname.lastname@example.org, email@example.com
ABSTRACT / САЖЕТАК:
Fungi could absorb heavy metals, metalloids, or radionuclides, thus fungal species possess great potential in bioremediation. Since fungi absorb the vanadium, in the present study ability of Coprinellus truncorum mycelia for vanadate uptake and its intracellular metabolism were investigated. The submerged cultivated C. truncorum was exposed to a rising concentration of vanadate. 31P NMR spectroscopy was used to investigate phosphate metabolism of the mycelium, while the status of vanadium in the cell was followed by 51V NMR spectroscopy. The mycelium could grow, and overcome vanadate presence, up to the concentration of 1.6 mM in the submerged medium. 31P NMR measurements pointed out that vanadate induced changes in the concentration of the crucial metabolite containing phosphorus, particularly sugar phosphates. The major result of vanadate action is evinced through an appearance of a signal positioned at around 2.8 ppm, and an increased signal of hexose- phosphates. Using 51V NMR spectroscopy the presence of vanadate monomer in the mycelia of the fungal cell was confirmed.
KEY WORDS / КЉУЧНЕ РЕЧИ:
fungi, phosphate metabolism, vanadium, 31P NMR spectroscopy, 51V NMR spectroscopy
REFERENCES / ЛИТЕРАТУРА:
- D., Mani, C., Kumar., Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation, International Journal of Environmental Science and Technology 11 (2014) 843-872.
- J.A., Campos, N.A., Tejera., Bioconcentration factors and trace elements bioaccumulation in sporocarps of fungi collected from quartzite acidic soils, Biological Trace Elements Research 143, (2011) 540-554.
- A., Levina, D.C., Crans, P.A., Lay., Speciation of metal drugs, supplements and toxins in media and bodily fluids controls in-vitro activities, Coordination Chemistry Reviews 352, (2017) 473- 498.
- D., Rehder., The role of vanadium in biology. Metallomics. 7, (2015) 730-742.
- J., Gordon., Use of vanadate as protein-phosphotyrosine phosphatase inhibitor, Methods in Enzymology 201, (2001) 477-482.
- M., Žižić, Z., Miladinović, M., Stanić, M., Hadžibrahimović, M., Živić, J., Zakrzewska., 51V NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium, Research in Microbiology 167, (2016) 521-528.
- M., Žižić, J., Zakrzewska, K., Tešanović, E., Bošković, M., Nešović, M., Karaman., Effects of vanadate on the mycelium of edible fungus Coprinus comatus, Journal of Trace Elements in Medicine and Biology 50, (2018) 320-326.
- M., Žižić, M., Živić, V., Maksimović, M., Stanić, S., Križak, T.C., Antić, J., Zakrzewska., Vanadate influence on metabolism of sugar phosphates in fungus Phycomyces blakesleeanus, PLoS One 9, (2014) 2-8.
- J., Benabe, L.A., Echegoyen, B., Pastranall, M., Martinez-Maldonado., Mechanism of inhibition of glycolysis by vanadate, Journal of Biological Chemistry 262 (1987) 9555-9560.