Savetovanje o biotehnologiji sa međunarodnim učešćem (Čačak: 27; 2022) (str. 477-482)

АУТОР(И): Monika Stojanova, Dragutin Djukic, Marina Todor Stojanova, Aziz Şatana, Blazo Lalevic


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DOI: 10.46793/SBT27.477S


The aim of this research was to determine the antibacterial potential ofaqueous and ethanolic extract of wild mushroom species: Agaricus macrosporus and Russulavesca. Extracts from R.vesca were characterized with higher values for total carbohydrates and total proteins. Both aqueous extracts had higher antibacterial activity compared to ethanolic extracts. Aqueous extract from R.vesca showed higher antibacterial activity against B. cereus (13.6mm), E.faecalis (12.1 mm), E. coli(16.7 mm) and P.aeruginosa (10.5 mm) compared to gentamicin or neomycin. This study represents a novel starting point for future researchin which mushroom extracts can be used in various industry fields.


wild mushrooms, extracts, antibacterialactivity.


  • Barros L., Cruz T., Baptista P., Estevinho L.M., Ferreira, I.C.F.R. (2008). Wild and commercial mushrooms as source of nutrients and nutraceuticals. Food and Chemical Toxicology, 46(8): 2742-2747. DOI:10.1016/j.fct.2008.04.030
  • Bradford M.M. (1976).A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Chemistry, 72: 248-254.
  • Chang S.T., Miles P.G. (2004). Mushrooms – Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact – 2nd ed., CRC Press.
  • Dahlberg A. (2019). Russulavesca. The IUCN Red List of Threatened Species 2019: e.T122090747A122091073.
  • Hleba L., Vuković N., Petrová J., Kačániová M. (2014). Antimicrobial Activity of Crude Methanolic Extracts from Ganodermalucidum and Trametesversicolor. Animal Science and Biotechnologies, 47(2): 89-93.
  • Iwalokun B.A., Usen U.A., Otunba A.A., Olukoya D.K. (2007). Comparative phytochemical evaluation, antimicrobial and antioxidant properties of Pleurotusostreatus. African Journal of Biotechnology 6(15): 1732-1739.
  • Jagadish L.K., Venkata V., Shenbhagaraman R., Kaviyarasan V. (2009). Comparitive study    on    the    antioxidant,     anticancer     and     antimicrobial     property  of Agaricusbisporus (J.E. Lange) imbach before and after  boiling. African Journal of Biotechology, 8(4): 654-661.
  • Klaus A., Kozarski M., Vunduk J., Todorovic N., Jakovljevic D., Zizak Z., Pavlovic V., Levic S., Niksic M., Van Griensven L.J.L.D. (2015). Biological potential of extracts of wild edible Basidiomycete mushroom Grifolaforndosa.Food Research International, 67: 272-283.
  • Loganathan J., Krishnan V.V., Ramalingam S., Kaviyarasan V. (2009). Comparative study on the antioxidant, anticancer and antimicrobial property of Agaricusbisporus (J. E. Lange) Imbach before and after boiling. African Journal of biotechnology, 8(4): 654-651.
  • Masuko T., Minami A., Iwasaki N., Majimab T., Nishimurac S.I., Lee Y.C. (2005). Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Analytical Biochemistry, 339: 69-72. http://doi:10.1016/j.ab.2004.12.001.
  • Mizuno M., Nishitani Y. (2013).Immunomodulating compounds in Basidiomycetes. Journal of Clinical Biochemistry and Nutrition, 52(3): 202–207.
  • Nwachukwu E., Uzoeto H.O. (2010). Antimicrobial activity of some local mushrooms on pathogenic isolates. Journal of Medicinal Plants Research, 4(23): 2460-2465. DOI: 10.5897/JMPR10.154
  • Reis F.S., Stojković D., Barros L., Glamočlija J., Ćirić A., Soković M., Martins A., Vasconcelos M.H., Morales P., Ferreira I.C.F.R. (2014). Can Suillusgranulatus (L.) Roussel be classified as a functional food? Food and Function, 5: 2861–2869. C4FO00619D
  • Ribeiro A., Ruphuy G., Lopes J.C., Dias M.M., Barros L., Barreiro F., Ferreira I.C.F.R. (2015). Spray-drying microencapsulation of synergistic antioxidant mushroom extracts and their use as functional food ingredients. Food Chemistry, 188: 612–618. https://doi. org/10.1016/j.foodchem.2015.05.061.
  • Santoyo S., Ramirez Anguiano A.C., Regiero G., Soler Rivas C. (2009). Improvement of the antimicrobial activity of edible mushroom extracts by inhibition of oxidative enzymes. International Journal of Food Science & Technology, 44(5):1057–1064. http://doi:10.1111/j.1365-2621.2008.01896.x.
  • Sławińska A., Radzki W., Kalbarczyk J. (2013). Antioxidant activities and polyphenolics content of Flammulinavelutipes mushroom extracts. KerlaPolonica, De Gruyter, 59(3): 26–36. https://doi. org/10.2478/hepo- 2013-0014.
  • Stojanova M., Pantić M., Karadelev M., Čuleva B., Nikšić M. (2021). Antioxidant potential of extracts of three mushroom species collected from the Republic of North Macedonia. Journal of Food Processing and Preservation.45(2): e15155. https://
  • Suntaxi T.C., Loja S.S. (2021). Antibacterial activity of the Agaricuspampeanus (Agaricaceae) ethanol extract against Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. ActualidadesBiológicas. 42(113): 1-10. DOI:10.17533/udea.acbi.v42n113a03
  • Tian Y., Zeng H., Xu Z., Zheng B., Lin Y., Gan C., Lo Y.M.(2012). Ultrasonicassisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom (Agaricusbisporus). Carbohydr.Polym.88: 522– 529.
  • Vidović S., Zoran-Zeković Z., Mujić I., LepojevićŽ.,Radojković M., Živković J. (2011). The antioxidant properties of polypore mushroom Daedaleopsisconfragosa. Central European Journal of Biology, 6(4): 575–582.
  • Wasser S.P. (2011).Current findings, future trends, and unsolved problems in studies of medicinal mushrooms. Applied Microbiology and Biotechnology, 89: 1323–1332.