1st International Conference on Chemo and BioInformatics, ICCBIKG  2021, (470-473)

AUTHOR(S) / АУТОР(И): Dragica Božić, Katarina Živančević, Katarina Baralić, Dragana Javorac, Aleksandra Buha Đorđević, Evica Antonijević Miljaković, Đurđica Marić, Marijana Ćurčić, Zorica Bulat, Biljana Antonijević, Danijela Đukić-Ćosić


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DOI: 10.46793/ICCBI21.470B


The aim of this study was to predict the molecular mechanisms and pathways of immunomodulator sulforaphane (SFN) against carcinoma using in silico toxicogenomic data mining. Three key tools applied in our analysis were Comparative Toxicogenomics Database (CTD; http://CTD.mdibl. org), ToppGene Suite portal ( and Reactome Knowledgebase ( Sulforaphane interacted with a total of 1896, among which NFE2L2, NQO1, HMOX1, GCLC, TXNRD1, IL1B, IFNG, AGT, KEAP1, and CASP3 had the highest number of interactions. In the CTD, there were direct evidences that SFN interacts with a total of 169 genes to express a therapeutic effect against different types of cancer such as: hepatocellular carcinoma (113), colorectal neoplasms (67), uterine cervical neoplasms (10), and adenomatous polyposis coli (4). This set of genes was further uploaded into the Gene Mania software, ToppGene Suite portal, and Reactome Knowledgebase, which confirmed that molecular functions, biological processes and pathways of SFN-affected genes were mostly related to oxidoreductase activity, regulation of immune system, and apoptosis. In conclusion, we may suggest that SFN interacts with host immunity to enhance the eradication of tumor cells mainly by inducing immune-response and stimulating apoptotic process of tumor cells. Moreover, its antioxidative activity could contribute to better anti-cancerogenic effects.


bioinformatics, carcinoma, sulforaphane, data mining, toxicogenomics


  • P. A. Ganz, Current US Cancer Statistics: Alarming Trends in Young Adults?, Journal of the National Cancer Institute. 111 (2019) 1241–1242.
  • K. D. Miller, L. Nogueira, A.B. Mariotto, J.H. Rowland, K.R. Yabroff, C.M. Alfano, A. Jemal, J.L. Kramer, R.L. Siegel, Cancer treatment and survivorship statistics, 2019, CA: A Cancer Journal for Clinicians. 69 (2019) 363–385.
  • G. Wu, Y. Yan, Y. Zhou, Y. Duan, S. Zeng, X. Wang, W. Lin, C. Ou, J. Zhou, Z. Xu, Sulforaphane: Expected to Become a Novel Antitumor Compound, Oncology Research. 28 (2020) 439–446.
  • M. Lenzi, C. Fimognari, P. Hrelia, Sulforaphane as a Promising Molecule for Fighting Cancer, Advances in Nutrition and Cancer. (2014) 207–23.
  • Q. Meng, J. Richmond-Bryant, S.E. Lu, B. Buckley, W.J. Welsh, E.A. Whitsel, A. Hanna, K.B. Yeatts, J. Warren, A.H. Herring, A. Xiu, Cardiovascular outcomes and the physical and chemical properties of metal ions found in particulate matter air pollution: A QICAR study, Environmental Health Perspectives. 121 (2013) 558–564.
  • M. Franz, H. Rodriguez, C. Lopes, K. Zuberi, J. Montojo, G.D. Bader, Q. Morris, GeneMANIA update 2018, Nucleic Acids Research. 46 (2018) W60–W64.
  • J. Chen, E.E. Bardes, B.J. Aronow, A.G. Jegga, ToppGene Suite for gene list enrichment analysis and candidate gene prioritization, Nucleic Acids Research. 37 (2009) 305–311.
  • B. Jassal, L. Matthews, G. Viteri, C. Gong, P. Lorente, A. Fabregat, K. Sidiropoulos, J. Cook, M. Gillespie, R. Haw, F. Loney, B. May, M. Milacic, K. Rothfels, C. Sevilla, V. Shamovsky, S. Shorser, T. Varusai, J. Weiser, G. Wu, L. Stein, Hermjakob, P. D’Eustachio, The reactome pathway knowledgebase, Nucleic Acids Research. 48 (2020) D498– D503.
  • F. Yang, F. Wang, Y. Liu, S. Wang, X. Li, Y. Huang, Y. Xia, C. Cao, Sulforaphane induces autophagy by inhibition of HDAC6-mediated PTEN activation in triple negative breast cancer cells, Life Sciences. 213 (2018) 149–157.
  • Y. Xia, T.W. Kang, Y. Do Jung, C. Zhang, S. Lian, Sulforaphane Inhibits Nonmuscle Invasive Bladder Cancer Cells Proliferation through Suppression of HIF-1α-Mediated Glycolysis in Hypoxia, Journal of Agricultural and Food Chemistry. 67 (2019) 7844–7854.
  • L. Gamet-Payrastre, P. Li, S. Lumeau, G. Cassar, M.A. Dupont, S. Chevolleau, N. Gasc, J. Tulliez, F. Tercé, Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells, Cancer Research. 60 (2000) 1426–1433.
  • J. Zhu, S. Zhang, J. Jiang, X. Chen, Definition of the p53 functional domains necessary for inducing apoptosis, Journal of Biological Chemistry. 275 (2000) 39927–39934.
  • A. Scoumanne, S.J. Cho, J. Zhang, X. Chen, The cyclin-dependent kinase inhibitor p21 is regulated by RNA-binding protein PCBP4 via mRNA stability, Nucleic Acids Research. 39 (2011) 213–224.